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Introduction
Components | Shipping Condition | Unit Size | Product Line | Detection Method |
E. coli DNA Control | -20℃ | 100 Reactions | SHENTEK™ | Probe-based qPCR |
qPCR Master MIX | ||||
DNA Dilution Buffer (DDB) | ||||
E. coli Primer&Probe MIX (Incl IPC) | -20℃, protect from light |
Overview
SHENTEK™ Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Specification
Range | 3x10-2-3x102 pg/μL, R2=0.99968, AE=97.5% |
Accuracy | Recovery = 81.2%-107.4% , CV<30% |
LOQ | 0.03 pg/μL |
LOD (LLOQ) | 0.003 pg/μL |
Precision | 2.2%-17.9% (<30%) |
Robustness | Freeze-thaw stability over 5 cycles |
Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments |
Related Products
SHENTEK™ Residual Host Cell DNA Sample Preparation Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.