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$370.00
Sku: TL-766-0100
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by April 28, 2026
Description
Laminin-511 E8 is a recombinant truncated protein expressed in HEK-293 cells and purified via affinity chromatography, comprising the C-terminal domains of α5, β1, and γ1 chains while retaining full integrin-binding capacity.
As a critical component of the basement membrane, Laminin-511 E8 provides structural support for pluripotent stem cells (PSCs) through its cell-binding motifs (e.g., integrin-binding sites), significantly enhancing cell adhesion, proliferation, and undifferentiated state maintenance.
Studies demonstrate that compared to full-length Laminin-511 or traditional matrices (e.g., Matrigel), Laminin-511 E8 exhibits superior cell adhesion efficiency and supports long-term stable passaging (>10 passages) of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) without karyotypic abnormalities.
Its optimized design enables dual applications in pre-coating and direct suspension mixing methods, offering a defined and efficient solution for organoid culture and directed differentiation research.
Specifications
| Specification | Acceptance Criteria | Results |
|---|---|---|
| SDS-PAGE | The electrophoretic bands should be consistent with the molecular weight of the product | Complies with specifications |
| Appearance | Freeze-dried powder; white, loose solid | Complies with specifications |
| Visible Foreign Matter | No visible foreign particles | Complies with specifications |
| Moisture Content | ≤ 4.0% | 0.5% |
| pH | 6.5 – 7.5 | 7.5 |
| Purity | > 90% | Complies with specifications |
| Bacterial Endotoxin | < 0.1 EU/μg | < 0.1 EU/μg |
| Mycoplasma | Negative | Negative |
| Sterility | No bacterial growth | No growth observed |
| Biological Activity | Promotes hiPSC adhesion with ED50 ≤ 5 μg/mL; supports stable hiPSC passaging | ED50 = 1 μg/mL |
Features
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✔
Optimized E8 Fragment Design
Retains full integrin-binding activity with reduced structural complexity for higher consistency than full-length laminin.
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Superior hiPSC Adhesion Efficiency
Demonstrates strong cell adhesion potency (ED₅₀ ≈ 1 µg/mL), outperforming traditional matrices such as Matrigel.
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✔
Supports Long-Term Pluripotency
Enables stable maintenance and passaging (>10 passages) of hESCs and iPSCs without karyotypic abnormalities.
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✔
Defined, Recombinant Matrix
HEK293 expression and affinity purification provide a xeno-free, well-defined substrate for reproducible stem cell culture.
Applications
iPSC / hESC Maintenance
Enhances adhesion, proliferation, and undifferentiated state maintenance in feeder-free culture systems.
Organoid Culture Systems
Suitable for both surface coating and direct mixing methods in 3D organoid workflows.
Directed Differentiation Studies
Provides a controlled extracellular matrix for lineage-specific differentiation research.
Stem Cell Manufacturing Research
Ideal for standardized and scalable pluripotent stem cell expansion protocols.
Stability & Storage
- Lyophilized powder: Store at −20 °C in the original sealed container.
- Handling after reconstitution: Prepare solutions under sterile conditions. Store reconstituted protein according to experimental requirements and avoid repeated freeze–thaw cycles.
- General handling: Ensure solutions are free of contamination to maintain biological activity.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Safety: Use appropriate PPE and standard laboratory safety practices when handling this product.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
