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Specification
| Package | 1/PK/5/PK/10/PK |
| Full name | Ni-NTA 6FF(Pre-Packed Gravity Column),5mL |
| Applications | Purification of His labeled proteins from various expression sources such as Escherichia coli, yeast, insect cells, and mammalian cells. |
| Summary | The matrix of this medium has an average particle size of 90 μm 6% highly cross-linked agarose of m is chemically fixed to the hydroxyl groups on the surface of agarose using iminodiacetic acid (IDA) molecules, which are then complexed with metal Ni2+by IDA. The purification principle is based on the fact that the imidazole ring labeled with recombinant protein histidine can form stable chemical coordination bonds with transition metal Ni2+ions, thus being able to specifically, firmly, and reversibly adsorb onto the agarose matrix fixed with these metal ions. |
| Features | Compared to Ni IDA agarose made from trivalent metal ion chelating agent iminodiacetic acid, the non-specific adsorption of Ni NTA agarose is significantly reduced, allowing users to obtain higher purity target proteins; 2. Support column refolding of denatured inclusion body proteins, achieving the effect of simultaneous separation and refolding; 3. Can tolerate a certain concentration of reducing reagents（ β- Mercaptoethanol can be used up to 20 mM; 4. The agarose matrix remains stable within the pH range of 2-14 and can withstand repeated in situ cleaning (CIP); 5. Minimal leakage of Ni2+during the purification process; 6. It can be reused and regenerated 5-10 times. |
| Composition | 5 mL (1/5/10 columns) |
| Substrate | 6% agarose |
| Average Particle Size | 0 |
| Particle Size | 45 μm~165 μm |
| Protein Binding Ability | Approximately 10-40 mg histidine labeled protein/1 mL resin |
| Maximum Flow Rate | 150 cm/h |
| Pressurization | 0.3 MPa, 3 bar |
| Storage Buffer | 20% ethanol |
| Component | 5 mL (1/5/10 columns) |
Storage
2~8°C, do not freeze
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310131_P310132_P310129_P310127_EN_P.pdf
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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