2xYT Top Agar-Semi-Solid Medium

Product Description:

2xYT Top Agar is a semi-solid medium specially formulated for phage plaque assays and M13 bacteriophage propagation. Unlike standard 2xYT Agar, which forms firm solid plates, 2xYT Top Agar contains a lower concentration of agar (7 g/L), resulting in a softer gel that allows efficient diffusion and infection of bacteriophage within E. coli cultures.

This softer texture makes 2xYT Top Agar ideal for overlay techniques used in phage display, plaque assays, and recombinant phage studies, where clear plaque formation is essential for analyzing phage infectivity and replication efficiency.

Key Features:

• Semi-solid medium optimized for phage plaque formation.

• Specifically designed for M13 phage and E. coli systems.

• Facilitates efficient phage diffusion without compromising host viability.

• Ideal for phage display, plaque assays, and phage propagation workflows.

How It Differs from Standard 2xYT Agar:

• Agar Content: 7 g/L vs. 15 g/L (standard 2xYT Agar).

• Texture: Soft, allowing phage movement vs. firm solid for general bacterial growth.

• Application: Phage-specific overlays vs. general E. coli cultivation.

Composition (per liter):

• Yeast Extract: 10.0 g

• Tryptone: 16.0 g

• Sodium Chloride: 5.0 g

• Agar: 7.0 g

Usage: 38 g/L
pH (7.6 g/200 ml water @25°C): 6.6 – 7.0
Storage: Room temperature. Protect from moisture.
Shipping Conditions: RT
UNSPSC Code: 41106200 – Microbiological Media
Product Code: SD7021
Packaging: 250 g/container

Ideal for Researchers Working With:

• Filamentous phages such as M13.

• Phage display systems in molecular biology.

• Plaque assay analysis for quantifying phage titer.

Documents

COA