FnCas12a, Active

Recombinant Francisella tularensis FnCas12a protein with a His tag expressed in E. coli. CRISPR enzyme sold with 1ml of Cas12 reaction buffer, 10x (CS12-09).

$117.60

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Sku: CF12A-E311H-50
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Description

CRISPR (clustered regularly interspaced short palindromic repeat) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development (3). Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. FnCas12a from Francisella shows increased stability at >37◦C making it ideal for LAMP (loop-mediated isothermal amplification) (4).

Accession Number

WP_003040289

Formulation

Recombinant protein stored in 25mM Tris, 300mM NaCl pH7.5 50% glycerol 0.1mM TCEP.

Documents

FnCas12a, Active

References

  • Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.

  • Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.

  • Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.

  • Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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