Deoxycholic Acid, Sodium Salt 98% High Purity Grade

Description

An ionic detergent for the solubilization of membrane-bound proteins (aggregation number: 4-10; CMC: 4-8 mM; micellar weight: 1700-4200; pKa 6.2). Reported to induce tyrosine phosphorylation of β-catenin and its subsequent translocation to the nucleus, promoting activation of β-catenin signaling (effective concentration 5-50 µM).

Specifications

Features

• High Assay Purity (HPLC): Verified at ≥ 98.0% purity, ensuring minimal experimental variability and highly reproducible data.

• Excellent Membrane Solubilization: Exhibits reliable micellar properties with an aggregation number of 4–10, a CMC of 4–8 mM, and a micellar weight of 1700–4200.

• Exceptional Quality Benchmarks: Strictly controlled to ensure low background impurities, with sodium cholate ≤ 2.0% and heavy metals ≤ 20 ppm. 

• Reliable Dissolution: Dissolves completely to form a perfectly clear solution (tested as a 2% autoclaved solution).

• Convenient Storage & Non-DG Shipping: Completely non-regulated for transport and safely stored at room temperature (18 to 25 ℃), reducing your shipping overhead.

Applications

• Membrane Protein Extraction: Extensively used as an ionic surfactant to solubilize cell membranes and isolate membrane-bound receptor proteins.

• Cell Signaling Research: Utilized at effective working concentrations of 5–50 µM to stimulate tyrosine phosphorylation and activate downstream β-catenin nuclear translocation pathways.

• Cell Lysis Buffers: Serves as a core component in the formulation of RIPA lysis buffers and other cell fractionation protocols.

How to Use & Preparation

• Solution Preparation: Dissolve the white-to-off-white powder in deionized or distilled water to achieve your desired working concentration (e.g., a standard 2% w/v stock solution).

• Sterilization: If sterile conditions are required for cell culture applications, the prepared solution can be safely autoclaved, resulting in a clear, stable liquid.

• Moisture Protection: Always keep the original bulk container tightly closed immediately after sampling to protect the hygroscopic powder from atmospheric moisture. 

Documents

COA

SDS

FAQ

Q1: What is the purity level and common grade of this sodium deoxycholate?
A: This product is supplied as a High Purity Grade reagent with a minimum HPLC assay of ≥ 98.0%, ensuring high reliability for cell lysis and protein isolation assays. 

Q2: Can I clear or sterilize the prepared solution by autoclaving?
A: Yes. Our product is tested for stability under heat; a 2% aqueous solution remains perfectly clear after being autoclaved.

Q3: How should Deoxycholic Acid Sodium Salt be stored?
A: It should be stored at room temperature (18 to 25 ℃) in a tightly sealed container. It is critical to protect the material from environmental moisture to prevent clumping or degradation.

Q4: Is this product considered a Dangerous Good (DG) for shipping?
A: No, this chemical is classified as non-hazardous under TDG, IMDG, and IATA transportation regulations, meaning it does not require special hazardous material handling fees during shipping.

Q5: What are its critical micellar properties as a biochemical detergent?
A: It is an ionic detergent characterized by a Critical Micelle Concentration (CMC) of 4–8 mM, an aggregation number of 4–10, and a micellar weight ranging from 1700 to 4200.