0 item(s)
View cart
You have no items in your shopping cart.
$47.00
Sku: B2217-1
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 25, 2026
Description
Blue Dextran 2000 (CAS 87915-38-6) is a high-molecular weight polymer (average MW ~2,000,000 Da) covalently conjugated with Cibacron Blue dye, widely used as a premium void volume (V0) marker and molecular weight standard in size-exclusion chromatography (SEC).
Specifications
| Property | Specification |
|---|---|
| Product Name | Blue Dextran 2000 |
| CAS Number | 87915-38-6 |
| Average Molecular Weight | ~2,000,000 Da |
| Appearance | Blue to dark blue powder or crystalline solid |
| Substitution Degree | ~0.06 mmol/g dye |
| Loss on Drying | ≤ 3.0% |
| Storage Temperature | Ambient temperature (15°C – 30°C), keep tightly sealed and protected from light |
| Shipping Condition | Ambient |
Features
-
Ideal Void Volume Marker: The massive molecular weight (~2,000,000 Da) ensures complete exclusion from most gel filtration matrix pores.
-
High Visual Contrast: Covalently bound to a triazinyl dye (Cibacron Blue F3GA) for easy visual tracking and precise UV/Vis detection (wavelength maximum around 620 nm).
-
Excellent Water Solubility: Dissolves quickly and completely in standard aqueous buffers without requiring any organic solvents.
-
Biofargo Quality Assured: Strict batch-to-batch consistency with low loss on drying (≤ 3%) to ensure reproducible chromatographic runs.
-
Reliable Supply Chain: Available in multiple packing sizes, stocked and shipped directly from our warehouse to keep your lab running smoothly.
Applications
-
Size-Exclusion & Gel Filtration Chromatography (SEC): Accurately determine the void volume (V0) of columns, evaluate packing efficiency, and check bed consistency.
-
Column Calibration & QC: Serves as a reliable high-molecular-weight reference standard to calibrate chromatography systems across different runs.
-
Permeability & Barrier Integrity Assays: Ideal for investigating macromolecular transport, diffusion, and epithelial/endothelial monolayer permeability due to its large size.
-
Protein Affinity Studies: Utilizes the Cibacron Blue dye moiety to bind specific proteins, allowing fast separation via void-volume elution.
How to Use
-
Preparation: Dissolve Blue Dextran 2000 in your running buffer to a final typical concentration of 1–2 mg/mL. Ensure it is fully dissolved.
-
Sample Clarification: Filter the solution through a 0.22 micron or 0.45 micron membrane filter before injection to protect your column from particulates.
-
Sample Loading: Apply an injection volume equivalent to 1% to 3% of the total column bed volume (Vt) for optimal peak sharpness.
-
Detection: Monitor the elution visually or via a UV-Vis detector set between 600 nm and 640 nm (optimal wavelength maximum around 620 nm).
Safety
Research Use Only: This product is intended solely for laboratory research and development. Not for human, drug, or veterinary use.
-
Always wear appropriate Personal Protective Equipment (PPE) including safety glasses, lab coats, and gloves during handling.
-
Avoid dust inhalation and direct skin contact.
-
For complete hazard, handling, and disposal instructions, please refer to the Biofargo Safety Data Sheet (SDS).
Documents
FAQ
Q1: Why is my Blue Dextran 2000 peak tailing or broader than expected?
A: This can occur if the sample is too concentrated or viscous. Try reducing the concentration to 1 mg/mL or decreasing the injection volume. Ensure the column is properly packed.
Q2: How do I prepare a stock solution and store it?
A: Reconstitute in your preferred aqueous chromatography buffer. Prepared solutions can be stored at 4°C for short-term use, but sterile filtration or adding a preservative (like sodium azide) is recommended to prevent microbial growth.
Q3: Can Blue Dextran 2000 be used in any chromatography column?
A: It is ideal for size-exclusion chromatography matrixes with exclusion limits below 2,000,000 Da. For columns with extremely large pore sizes (e.g., some ultra-large pore acrylic beads), it may partially enter the matrix.
Q4: Is Blue Dextran 2000 stable at room temperature?
A: Yes, the powder form is highly stable at ambient temperature when stored in a dark, dry environment. Always keep the container tightly closed.
Q5: How do I request bulk quantities or custom packaging?
A: Biofargo provides bulk scale packaging for industrial and pilot studies. Please click our "Request Bulk Quote" button or contact our sales team directly for tiered pricing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
