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Double-Layer Design • Light-Protective Option
Description
NO MORE FOIL. The all-black PP body blocks light completely - protect your fluorescent antibodies without wrapping (and re-wrapping, and tearing) aluminum foil ever again
ONE BOX = 8 COMPARTMENTS, ONE SHAKER SPOT. Six strip channels + two full-membrane wells in a hand-sized 13.5 x 8.5 cm footprint. Stop letting one primary incubation take over two shakers.
SAVE EXPENSIVE ANTIBODY. Narrow 2.3 x 8.3 cm channels fully cover a strip with just 1-2 mL of antibody dilution - and let you probe 6 different targets from one gel, at once.
SPILL-PROOF LATCH LID. The red locking clip seals the lid tight: shake it, tilt it, carry it to the cold room - no spills, less evaporation on overnight incubations.
Specifications
| Specification | Detail |
| Overall dimensions | 13.5 x 8.5 x 4.5 cm (5.3" x 3.3" x 1.8") |
| Upper layer | 6 strip channels, 2.3 x 8.3 cm (0.9" x 3.3") each |
| Lower layer | 2 membrane wells, 6.4 x 8.4 cm (2.5" x 3.3") each |
| Min. working volume | ~1-2 mL per strip channel; ~4-6 mL per membrane well |
| Material | Polypropylene (PP), chemical-resistant, non-binding surface |
| Colors | Black (light-protective) / Clear (see-through) |
| Pack size | 1 piece |
Features
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Double-layer compartment design
Separates incubation workflow into two independent levels, improving experimental organization and efficiency.
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Reduced antibody consumption
Optimized chamber design minimizes reagent volume required for membrane incubation and washing steps.
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Drainage outlet structure for easy liquid exchange
Drainage outlet design for easier liquid exchange and buffer removal and reduces residual liquid during washing steps.
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Chemical-resistant PP material
Made from high-quality polypropylene (PP), resistant to common laboratory reagents and suitable for repeated Western blot use.
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Transparent and light-protective options available
Available in clear version for direct visual monitoring and black version for light-sensitive applications such as chemiluminescence and fluorescence detection.
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6-compartment upper layer design
Supports parallel incubation of multiple membranes or antibody conditions for higher experimental throughput.
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2-compartment lower layer design
Provides larger-volume incubation or washing space for membranes requiring extended processing or higher reagent coverage.
Applications
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Western blot antibody incubation
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Membrane washing (TBST / PBST steps)
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Protein membrane staining and destaining (Western blot workflows)
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Chemiluminescent and fluorescence detection workflows
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Immunoblot signal development and optimization
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Western blot-based protein analysis workflows
Why This Box Matters in Western Blot Workflow
Western blot performance is not only determined by antibodies and detection systems, but also by every incubation and washing step.
This incubation box helps improve workflow handling in Western blot procedures:
Helps reduce variability caused by inconsistent washing conditions
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Supports more uniform buffer exchange during incubation
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Reduces handling disturbance of membranes
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Provides light protection in black version for sensitive workflows
By optimizing the physical environment of incubation, this box helps support consistent Western blot incubation and washing workflows.
Biofargo vs Typical Single-Well Blot Box
| Biofargo 8-Channel Box | Typical Single-Well Blot Box | |
| Compartments per box | 8 (6 strips + 2 membranes) | 1 |
| Cost per compartment | $2.40 (at $19/box) | $10+ (sold in 5-packs, $50+) |
| Antibody per strip | 1-2 mL | 4-6 mL or more |
| Material | Polypropylene (PP) | Usually polystyrene (PS) |
| Minimum purchase | 1 piece | Often 5-pack only |
Which Color Should I Choose?
Black - Light-protective. Choose for fluorescent western blot (LI-COR, Odyssey, iBright), fluorophore-conjugated secondary antibodies, and any light-sensitive incubation.
Clear - See-through. Choose for standard chemiluminescent WB, staining, and washing steps where you want to monitor your membrane and solutions at a glance.
Many labs keep both: clear for daytime washes, black for overnight fluorescent incubations.
Storage & Handling
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Store at room temperature in a clean, dry environment
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Avoid strong organic solvents unless compatibility is confirmed
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Clean with mild detergent or laboratory-grade cleaning solutions
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Do not expose to prolonged UV or harsh chemical sterilization unless validated
FAQ
What is a Western blot incubation box used for?
What is the advantage of a smooth PP surface in this box?
Can this box be used for fluorescence detection workflows?
Is this suitable for PVDF and nitrocellulose membranes?
Can it be reused?
Is the black box completely light-proof?
Can I run different primary antibodies in adjacent channels?
Is it dishwasher-safe?
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

