Double-Layer Design • Light-Protective Option

$19.00

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Sku: AC2123
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by August 26, 2026

Description

NO MORE FOIL. The all-black PP body blocks light completely - protect your fluorescent antibodies without wrapping (and re-wrapping, and tearing) aluminum foil ever again

ONE BOX = 8 COMPARTMENTS, ONE SHAKER SPOT. Six strip channels + two full-membrane wells in a hand-sized 13.5 x 8.5 cm footprint. Stop letting one primary incubation take over two shakers.

SAVE EXPENSIVE ANTIBODY. Narrow 2.3 x 8.3 cm channels fully cover a strip with just 1-2 mL of antibody dilution - and let you probe 6 different targets from one gel, at once.

SPILL-PROOF LATCH LID. The red locking clip seals the lid tight: shake it, tilt it, carry it to the cold room - no spills, less evaporation on overnight incubations.

Specifications

Specification Detail
Overall dimensions 13.5 x 8.5 x 4.5 cm (5.3" x 3.3" x 1.8")
Upper layer 6 strip channels, 2.3 x 8.3 cm (0.9" x 3.3") each
Lower layer 2 membrane wells, 6.4 x 8.4 cm (2.5" x 3.3") each
Min. working volume ~1-2 mL per strip channel; ~4-6 mL per membrane well
Material Polypropylene (PP), chemical-resistant, non-binding surface
Colors Black (light-protective) / Clear (see-through)
Pack size 1 piece

Features

  • Double-layer compartment design

    Separates incubation workflow into two independent levels, improving experimental organization and efficiency.

  • Reduced antibody consumption

    Optimized chamber design minimizes reagent volume required for membrane incubation and washing steps.

  • Drainage outlet structure for easy liquid exchange

    Drainage outlet design for easier liquid exchange and buffer removal and reduces residual liquid during washing steps.

  • Chemical-resistant PP material

    Made from high-quality polypropylene (PP), resistant to common laboratory reagents and suitable for repeated Western blot use.

  • Transparent and light-protective options available

    Available in clear version for direct visual monitoring and black version for light-sensitive applications such as chemiluminescence and fluorescence detection.

  • 6-compartment upper layer design

    Supports parallel incubation of multiple membranes or antibody conditions for higher experimental throughput.

  • 2-compartment lower layer design

    Provides larger-volume incubation or washing space for membranes requiring extended processing or higher reagent coverage.

Applications

  • Western blot antibody incubation

  • Membrane washing (TBST / PBST steps)

  • Protein membrane staining and destaining (Western blot workflows)

  • Chemiluminescent and fluorescence detection workflows

  • Immunoblot signal development and optimization

  • Western blot-based protein analysis workflows

Why This Box Matters in Western Blot Workflow

Western blot performance is not only determined by antibodies and detection systems, but also by every incubation and washing step.

This incubation box helps improve workflow handling in Western blot procedures:

Helps reduce variability caused by inconsistent washing conditions

  • Supports more uniform buffer exchange during incubation

  • Reduces handling disturbance of membranes

  • Provides light protection in black version for sensitive workflows

By optimizing the physical environment of incubation, this box helps support consistent Western blot incubation and washing workflows.

Biofargo vs Typical Single-Well Blot Box

Biofargo 8-Channel Box Typical Single-Well Blot Box
Compartments per box 8 (6 strips + 2 membranes) 1
Cost per compartment $2.40 (at $19/box) $10+ (sold in 5-packs, $50+)
Antibody per strip 1-2 mL 4-6 mL or more
Material Polypropylene (PP) Usually polystyrene (PS)
Minimum purchase 1 piece Often 5-pack only

Which Color Should I Choose?

Black - Light-protective. Choose for fluorescent western blot (LI-COR, Odyssey, iBright), fluorophore-conjugated secondary antibodies, and any light-sensitive incubation.

Clear - See-through. Choose for standard chemiluminescent WB, staining, and washing steps where you want to monitor your membrane and solutions at a glance.

Many labs keep both: clear for daytime washes, black for overnight fluorescent incubations.

Storage & Handling

  • Store at room temperature in a clean, dry environment

  • Avoid strong organic solvents unless compatibility is confirmed

  • Clean with mild detergent or laboratory-grade cleaning solutions

  • Do not expose to prolonged UV or harsh chemical sterilization unless validated

FAQ

What is a Western blot incubation box used for?
It is used for antibody incubation, membrane washing, and staining steps during Western blot experiments, providing a stable and contamination-free environment.
What is the advantage of a smooth PP surface in this box?
Non-binding surfaces help reduce liquid retention on plastic surfaces to plastic walls, helping maintain higher signal intensity and lower background noise.
Can this box be used for fluorescence detection workflows?
Yes. The opaque light-protective version is designed for fluorescence and chemiluminescence applications to minimize signal degradation.
Is this suitable for PVDF and nitrocellulose membranes?
Yes. It is compatible with both PVDF and nitrocellulose membranes commonly used in Western blot experiments.
Can it be reused?
Yes, it is designed for repeated laboratory use when properly cleaned and maintained.
Is the black box completely light-proof?
Yes. Both the body and the lid are molded from opaque black polypropylene, blocking ambient light completely. Your fluorophore-conjugated antibodies stay protected throughout incubation, washing, and transport — no foil wrapping needed at any step. That's the point: replace the tear-prone foil ritual entirely.
Can I run different primary antibodies in adjacent channels?
Yes. Each of the 6 upper channels is fully independent with no cross-flow, so you can incubate 6 different antibodies side by side.
Is it dishwasher-safe?
The box is made of polypropylene (PP), which is dishwasher-safe and chemically resistant to routine WB buffers.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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