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Sku: 140-200
Categories: PCR

Intended use

This reverse transcriptase or RNA-dependent DNA polymerase is used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe.


Product Characteristics

  • The reverse transcriptase is a thermophilic type A polymerase (US patent pending) with optimal temperatures at 60-62°C (User Manual Tables 1-2).

  • The RTase is easily heat-inactivated at ≥90°C for 1min.

  • The enzyme efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of singleplex reaction.

  • The enzyme reversely-transcribes single digit copies of target RNA molecules consistently.

  • The concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 4).

  • The preferable PCR product size is ≤150bp.

  • It is particularly suitable to one-step real-time qualitative and quantitative RT-PCR.



User Manual

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.


Terms and Conditions

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