Thermophilic Reverse Transcriptase-Genetic Tools

Intended use

This reverse transcriptase or RNA-dependent DNA polymerase is used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe.

Product Characteristics

• The reverse transcriptase is a thermophilic type A polymerase (US patent pending) with optimal temperatures at 60-62°C (User Manual Tables 1-2).

• The RTase is easily heat-inactivated at ≥90°C for 1min.

• The enzyme efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of singleplex reaction.

• The enzyme reversely-transcribes single digit copies of target RNA molecules consistently.

• The concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 4).

• The preferable PCR product size is ≤150bp.

• It is particularly suitable to one-step real-time qualitative and quantitative RT-PCR.

Documents

User Manual