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Description
This product was purified from a plasmid cloned with a gene encoding Thermus aquaticus DNA polymerase and induced expression by E. coli. Taq DNA polymerase does not have 3'¡ú5' exonuclease correction activity, but has very low 5'¡ú3' exonuclease activity. Recombinant Taq DNA polymerase is the recommended choice for most PCR reactions, with a half-life of > 40 min at 95¡ãC. The mutation rate of each circulating Taq DNA polymerase < 2.2¡Á10-5. The above-mentioned Taq DNA polymerase is free of contamination by foreign nucleases and bacterial DNA, and has high amplification efficiency and good stability, making it suitable for routine PCR amplification.
Specification
| Parameter | Specification |
|---|---|
| Package | 1 KU/5 KU/200 U |
| Full name | Taq DNA Polymerase |
| Composition | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
| Reaction Buffer | 10X PCR Buffer |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol deoxyribonucleotides into a polynucleotide fraction in 30 min at 74¡ãC. |
| Concentration | 5 U/¦Ìl |
| Storage Buffer | 20 mM Tris-HCl (PH 8.0) £»0.1 mM EDTA,£»1 mM DTT£»0.5% Tween 20£»100 mM KCl£»0.5% NP-40£»50% glycerin. |
| Component | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
Features
There is no contamination of foreign nuclease and bacterial DNA, and the amplification efficiency is high and the stability is good.
Applications
Conventional PCR amplification, RT-PCR, and direct TA cloning of the product.
Storage
-20¡ãC
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

