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Sku: VS-02-HZ-13-L-G
Syringe Filter – Hydrophilic PVDF – 0.22 / 0.45 μm (Female Luer Lock + Male Luer Slip)
Description
Designed for high-performance biological sample preparation, protein solution clarification, and HPLC sample filtration, the Biofargo Hydrophilic PVDF Syringe Filters deliver excellent chemical resistance and low protein adsorption for sensitive laboratory workflows.
Featuring a hydrophilic PVDF membrane with outstanding mechanical strength and broad chemical compatibility, these syringe filters provide efficient particulate removal while maintaining high analyte recovery. The membrane’s low-binding properties make it particularly suitable for protein-based solutions, enzyme samples, and biological fluids where sample integrity is critical.
Built with a robust polypropylene housing and precision-molded structure, Biofargo PVDF syringe filters ensure stable pressure resistance and consistent filtration performance under demanding laboratory conditions. Available in multiple pore sizes and diameters, the filters support a wide range of analytical, biochemical, and pharmaceutical applications requiring reliable liquid clarification and contamination control.
Specifications
| Parameter | Specification |
|---|---|
| Product Name | Biofargo Hydrophilic PVDF Syringe Filter |
| Membrane Material | Hydrophilic PVDF (Polyvinylidene Fluoride) |
| Housing Material | Polypropylene (PP) |
| Support Layer | Polypropylene (PP) |
| Available Pore Sizes | 0.22 μm, 0.45 μm |
| Available Diameters | 13 mm, 25 mm, 33 mm |
| Filtration Area | 0.13 cm², 1 cm², 3.5 cm² |
| Connector Type | Luer Inlet / Outlet |
| Maximum Forward Pressure | 5 bar (72.5 psi) @20°C |
| Maximum Backward Pressure | 1 bar (14.5 psi) @20°C |
| Chemical Compatibility | pH 1–14 |
| Sterilization Compatibility | Autoclaving and irradiation resistant |
| Protein Binding | Low adsorption membrane |
| Bacterial Retention | >10⁷ cfu/cm² B. diminuta (ATCC 19146) |
| Endotoxin Level | <0.25 EU/mL |
| Integrity Testing | 100% integrity tested before release |
| Biosafety Standard | USP <88> Class VI |
| Manufacturing Environment | 100,000 Class Clean Room |
Ordering Information
| SKU | Pore Size | Diameter | Package Size | Sterilization |
|---|---|---|---|---|
| VS-02-HZ-13-L-G | 0.22 μm | 13 mm | 15 Unit/Pack | Sterile |
| VS-02-HZ-13-L-N | 0.22 μm | 13 mm | 15 Unit/Pack | Non-sterile |
| VS-04-HZ-13-L-G | 0.45 μm | 13 mm | 15 Unit/Pack | Sterile |
| VS-04-HZ-13-L-N | 0.45 μm | 13 mm | 15 Unit/Pack | Non-sterile |
| VS-02-HZ-25-L-G | 0.22 μm | 25 mm | 25 Unit/Pack | Sterile |
| VS-02-HZ-25-L-N | 0.22 μm | 25 mm | 25 Unit/Pack | Non-sterile |
| VS-04-HZ-25-L-G | 0.45 μm | 25 mm | 25 Unit/Pack | Sterile |
| VS-04-HZ-25-L-N | 0.45 μm | 25 mm | 25 Unit/Pack | Non-sterile |
| VS-02-HZ-33-L-G | 0.22 μm | 33 mm | 30 Unit/Pack | Sterile |
| VS-02-HZ-33-L-N | 0.22 μm | 33 mm | 30 Unit/Pack | Non-sterile |
| VS-04-HZ-33-L-G | 0.45 μm | 33 mm | 30 Unit/Pack | Sterile |
| VS-04-HZ-33-L-N | 0.45 μm | 33 mm | 30 Unit/Pack | Non-sterile |
Features
- Hydrophilic PVDF Membrane Structure — provides excellent chemical resistance and stable filtration performance across diverse laboratory applications.
- Low Protein Adsorption — minimizes sample loss and preserves protein integrity during biological and biochemical workflows.
- High Mechanical Strength Membrane — ensures durability under pressure and reliable performance in demanding filtration conditions.
- Broad Chemical Compatibility (pH 1–14) — suitable for a wide range of aqueous and mildly organic laboratory solutions.
- Reliable Bacterial Retention — effective removal of microorganisms and particulates for sterile filtration applications.
- Robust Polypropylene Housing — ensures structural stability and leak-free operation during syringe-based filtration.
- Autoclave and Irradiation Resistant — compatible with standard laboratory sterilization procedures.
- Multiple Sizes and Pore Options — supports flexible use in HPLC, protein analysis, buffer preparation, and general laboratory filtration.
Applications
- Protein and Enzyme Filtration — low adsorption PVDF membrane preserves biomolecule activity and sample integrity.
- HPLC Sample Preparation — removes particulates to protect chromatographic columns and improve analytical accuracy.
- Biological Sample Clarification — suitable for serum, culture supernatant, and aqueous biological fluids.
- Buffer and Reagent Filtration — ensures clean, particle-free laboratory solutions for downstream experiments.
- Pharmaceutical and Analytical Filtration — supports high-purity workflows in research and quality control laboratories.
Frequently Asked Questions
What is a hydrophilic PVDF syringe filter used for?
A hydrophilic PVDF syringe filter is designed for filtration of aqueous laboratory samples where low protein adsorption and high sample recovery are important. It is widely used for protein solution filtration, biological sample clarification, buffer filtration, enzyme solutions, cell culture supernatants, and HPLC sample preparation. Unlike hydrophobic membranes, hydrophilic PVDF can be used directly with aqueous solutions without pre-wetting, helping simplify routine laboratory workflows.
Should I choose a 0.22 μm or 0.45 μm PVDF syringe filter?
The appropriate pore size depends on your application. A 0.22 μm PVDF syringe filter is commonly selected for sterile filtration of aqueous solutions and removal of bacteria and fine particulates before downstream biological or analytical experiments. A 0.45 μm PVDF syringe filter is generally preferred for sample clarification, HPLC sample preparation, and removal of larger suspended particles when sterile filtration is not required. Choosing the correct pore size helps balance filtration efficiency, flow rate, and membrane life.
Why is hydrophilic PVDF recommended for protein solution filtration?
Hydrophilic PVDF membranes feature low protein binding and low non-specific adsorption, helping reduce sample loss during filtration. This makes them well suited for protein-rich samples, enzymes, antibodies, recombinant proteins, and biological fluids where maintaining high protein recovery and sample integrity is important for downstream analysis.
Can these PVDF syringe filters be used for HPLC sample preparation?
Yes. These PVDF syringe filters for HPLC sample preparation efficiently remove fine particulates before sample injection, helping protect HPLC columns, reduce the risk of column blockage, improve chromatographic reproducibility, and extend analytical instrument life. Hydrophilic PVDF membranes are particularly suitable for aqueous samples and many commonly used laboratory solutions.
Do hydrophilic PVDF syringe filters require pre-wetting before use?
No. Hydrophilic PVDF membranes are designed for direct filtration of aqueous solutions and generally do not require pre-wetting before use. This allows faster sample preparation while minimizing handling steps and reducing the possibility of sample contamination during routine laboratory filtration.
What sample types are suitable for hydrophilic PVDF syringe filters?
Hydrophilic PVDF syringe filters are suitable for protein solutions, enzyme preparations, biological samples, tissue culture media, cell culture supernatants, aqueous buffers, laboratory reagents, and analytical samples requiring particulate removal before HPLC, LC, or other downstream applications.
How do I select the correct filter diameter: 13 mm, 25 mm, or 33 mm?
Filter diameter mainly affects filtration area and sample loading capacity. A 13 mm PVDF syringe filter is commonly used for small-volume samples. A 25 mm PVDF syringe filter is suitable for most routine laboratory applications and offers a good balance between flow rate and capacity. A 33 mm PVDF syringe filter provides the largest effective filtration area and is often preferred for larger sample volumes or solutions containing more suspended particles.
What is the difference between sterile and non-sterile PVDF syringe filters?
Sterile PVDF syringe filters are intended for applications requiring sterile filtration, including tissue culture media, biological solutions, and other contamination-sensitive laboratory workflows. Non-sterile PVDF syringe filters are commonly used for HPLC sample preparation, buffer filtration, reagent clarification, and general laboratory filtration where sterility is not required.
What chemicals are compatible with hydrophilic PVDF membranes?
Hydrophilic PVDF membranes provide broad chemical compatibility across a wide pH range (approximately pH 1–14) and are compatible with many aqueous laboratory solutions and selected organic solvent mixtures. For highly aggressive solvents or unusual chemical systems, compatibility testing is recommended before routine use.
What are the benefits of the Female Luer Lock and Male Luer Slip connection?
The Female Luer Lock inlet provides a secure, leak-resistant connection with standard laboratory syringes, while the Male Luer Slip outlet allows convenient transfer of filtered samples into collection tubes, sample vials, or analytical containers. This standardized connection design helps improve operational safety and supports reliable laboratory sample filtration.
Can these syringe filters help improve analytical accuracy?
Yes. Removing fine particles before analysis helps reduce instrument contamination, minimizes injection-related issues, improves sample consistency, and supports more reliable analytical results. This makes hydrophilic PVDF syringe filters a common choice for chromatography sample filtration, pharmaceutical research, biotechnology laboratories, and quality control applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
