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Specification
|
Full name |
Super Phi29 DNA Polymerase |
| Applications | 1. Rolling Ring Amplification (RCA); 2. Multiple Displacement Amplification (MDA); 3. DNA amplification for SNP and STR detection; 4. Whole genome amplification of single cells, pathogenic microorganisms, and metagenomes; 5. DNA amplification in blood spot samples from filter paper; 6. DNA template preparation for sequencing; 7. RNA and protein primers DNA amplification; 8. Unbiased amplification of the whole genome; 9. Cell-free cloning of lethal DNA; 10. High-precision DNA synthesis. |
| Summary | Super Phi29 DNA Polymerase is derived from recombinant E. coli carrying the Bacillus subtilis phage Phi29 gene, which was expressed by E. coli and isolated and purified multiple times. Phi29 DNA polymerase has high continuous synthesis capacity and strand replacement activity, and can continuously synthesize up to 70 kb of DNA fragments to achieve efficient isothermal DNA amplification. It has 3'-5' exonuclease (proofreading) activity that preferentially acts on single-stranded DNA or RNA, ensuring high fidelity of amplification reactions, and is commonly used in vitro preparation and genome-wide synthesis of plasmids. It has higher amplification efficiency and sensitivity compared to wild-type Phi29 DNA polymerase, and DNA synthesis can be performed continuously at 42°C (whereas wild-type Phi29 DNA polymerase has low reactivity at this temperature). |
| Features | 1. Highest processability and strand displacement activity of any known DNA polymerase – more than 70 kb of DNA fragments can be synthesized; 2. High-precision DNA synthesis; 3. Extremely high yields of amplified DNA can be obtained even from very small amounts of template; 4. Amplification products can be used directly for downstream applications (PCR, restriction digestion, SNP genotyping, etc.). |
| Composition | Super Phi29 DNA Polymerase,（10 U/μl） 10X Super Phi29 Buffer 100 mM DTT. |
| Reaction Buffer | 10X Super Phi29 Buffer |
| Unit Definition | 1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 minutes at 30°C. |
| Thermal Inactivation | Heat at 65 °C for 10 min to lose activity. |
| Concentration | 10 U/μl |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) NP-40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol. |
| Applications | 1. Rolling Ring Amplification (RCA); 2. Multiple Displacement Amplification (MDA); 3. DNA amplification for SNP and STR detection; 4. Whole genome amplification of single cells, pathogenic microorganisms, and metagenomes; 5. DNA amplification in blood spot samples from filter paper; 6. DNA template preparation for sequencing; 7. RNA and protein primers DNA amplification; 8. Unbiased amplification of the whole genome; 9. Cell-free cloning of lethal DNA; 10. High-precision DNA synthesis. |
| Component | Super Phi29 DNA Polymerase,（10 U/μl） 10X Super Phi29 Buffer 100 mM DTT. |
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Storage
-20°C
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.Â
Email contact@biofargo.com if you have any requirements.
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