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$120.00
Sku: BF-SS-500
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by August 27, 2026
BioFargo Inc. · Ready-to-use · 500 mL
Western Blot Stripping Buffer (StripSure™)
Mild, ready-to-use antibody stripping buffer for complete removal in 20 minutes at room temperature—without heat or harsh chemicals.
StripSure™ efficiently removes primary and secondary antibodies from Western blot membranes while preserving membrane-bound target proteins for reliable re-probing. It is compatible with PVDF, nitrocellulose, and nylon membranes; simply immerse, incubate, rinse, and re-probe.
Performance testing confirmed removal of detectable signal from previously probed antibodies (anti-ATG7), while re-probing generated strong, specific signal with β-actin and phospho-STAT3 (Tyr705). Its gentle conditions support sensitive phospho-specific detection workflows.
20 minRoom temperature protocol
MildNo heat or reducing agents
3 membranesPVDF, nitrocellulose & nylon
Key Features & Benefits
- Efficient removal of primary and secondary antibodies
- Compatible with phospho-specific antibody re-probing
- Helps preserve membrane-bound target proteins across stripping cycles
- Ready-to-use liquid formulation—do not dilute
- 20-minute protocol at room temperature (20–25°C) with gentle agitation
- Suitable for chemiluminescent and fluorescent detection systems
Mild vs. Harsh Stripping: Which Do You Need?
Stripping buffers fall into two categories, and choosing the wrong one is a common reason a re-probe fails.
Harsh stripping uses SDS and β-mercaptoethanol at 50–70°C. It removes essentially any antibody, including high-affinity antibodies, but can also strip target protein from the membrane. Signal loss on a second probe can be measurable, particularly for low-abundance targets.
Mild stripping uses low pH and mild detergent at room temperature. It removes antibody without displacing membrane-bound protein, supporting sequential probing that is quantitative rather than qualitative.
StripSure is a mild stripping buffer. It requires no heat, β-mercaptoethanol, or reducing agents: 20 minutes at room temperature. Use it when the second blot must be comparable to the first, when re-probing phospho-epitopes, or when a low-abundance target cannot tolerate signal loss.
If you are stripping a very high-affinity antibody and preserving the target is not important, a harsh protocol may still be faster. For other applications, mild stripping is the appropriate default.
Western Blot Stripping Protocol (20 Minutes, Room Temperature)
Step-by-Step
- Initial wash — Rinse the membrane once with deionized water to remove residual detection reagents.
- Stripping — Gently mix StripSure™ until homogeneous (do not dilute), then completely immerse the membrane.
- Incubation — Incubate for 20 minutes at room temperature (20–25°C) with gentle agitation.
- Rinsing — Rinse three times with deionized water, or three times with TBST for sensitive applications.
- Re-equilibration — Equilibrate the membrane in TBST for 5 minutes.
- Re-probing — Proceed with primary antibody incubation, wash, secondary antibody incubation, wash, and detection.
Important: Keep the membrane fully submerged throughout stripping for consistent, even results.
Stripping and Re-Probing Phospho-Specific Blots
Phospho-specific targets are particularly vulnerable to signal loss under heated or reducing stripping conditions. StripSure’s room-temperature, non-reducing mild stripping protocol is intended to retain membrane-bound protein for subsequent phospho-specific re-probing.
Troubleshooting: When Stripping Doesn’t Work
| Observation | Most likely cause | What to do |
|---|---|---|
| Original signal is still visible | High-affinity antibody or membrane not fully immersed | Run another 20-minute cycle; ensure full immersion and continuous gentle agitation. |
| Second-probe signal is much weaker | Target protein was eluted, often after a prior harsh protocol | Use a mild protocol; for low-abundance targets, consider re-running the blot. |
| High background on re-probe | Insufficient washing or residual stripping buffer | Wash with TBST three times for 5 minutes each; re-block for at least 1 hour. |
| Blotchy or uneven signal | Membrane dried during incubation | Keep it wet throughout; move directly to washing after stripping. |
| Phospho signal is lost | A heated or reducing protocol was used | Use a room-temperature, non-reducing mild protocol for phospho-epitopes. |
| Nothing works after two cycles | The membrane reached its stripping limit | Do not strip the same membrane more than 2–3 times; re-run the blot. |
StripSure™ vs. Restore™ PLUS
| StripSure™ | Restore™ PLUS (46430) | |
|---|---|---|
| 500 mL price | $120 | $189.65 |
| Incubation conditions | 20 min, room temperature | 5–15 min, room temperature; 37°C for high-affinity antibodies |
| Heat required | No | 37°C for difficult-to-strip antibodies |
| Membrane compatibility | PVDF, nitrocellulose, nylon | Nitrocellulose, PVDF |
| Ready to use | Yes; no dilution | Yes |
Pricing and specifications as published by the manufacturer, August 2026.
Specifications
| Product Name | StripSure™ Western Blot Stripping Buffer |
|---|---|
| Brand | BioFargo Inc. |
| Format | Ready-to-use liquid (do not dilute) |
| Compatible Membranes | PVDF, nitrocellulose, nylon |
| Detection Systems | Chemiluminescent and fluorescent imaging |
| Incubation | 20 minutes at room temperature (20–25°C), gentle agitation |
| Antibody Compatibility | Primary and secondary antibodies, including phospho-specific antibodies |
| Rinse Options | Deionized water or TBST (recommended for sensitive applications) |
| Size / Volume | 500 mL bottle |
| Storage | Room temperature |
| Shelf Life | 1 year |
Validation Data
StripSure™ was validated for antibody removal using anti-ATG7 and for re-probing using β-actin and phospho-STAT3 (Tyr705).
Frequently Asked Questions
How long does Western blot stripping take?
StripSure requires a 20-minute incubation at room temperature with gentle agitation.Can you strip and re-probe a phospho-specific Western blot?
Yes. StripSure is a mild, room-temperature, non-reducing buffer designed for re-probing, including phospho-specific workflows.How many times can you strip the same membrane?
Typically no more than two to three times. Re-run the blot after that.Is StripSure a mild or harsh stripping buffer?
StripSure is a mild stripping buffer; it works at room temperature without heat, β-mercaptoethanol, or reducing agents.Does it work with nitrocellulose and PVDF?
Yes. It is compatible with PVDF, nitrocellulose, and nylon membranes.Do I need to re-block after stripping?
Yes. After stripping and washing, re-block the membrane for at least one hour before re-probing.What is the difference between mild and harsh stripping buffers?
Harsh buffers use SDS and β-mercaptoethanol at elevated temperatures. Mild buffers use low pH and mild detergent at room temperature to help preserve membrane-bound protein.When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
