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Sku: SLAN-48P
SLAN®-48P 48-Well Real-Time PCR System
Description
Laboratories running real-time PCR experiments with up to 48 reactions require a system that supports common PCR consumables, multiple fluorescence channels, stable temperature control, and different data-analysis modes. The SLAN®-48P is a 48-well real-time PCR system with a reaction volume range of 15–100 µL. It is compatible with 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates.
For multiplex real-time PCR workflows, the SLAN®-48P provides four standard fluorescence channels and supports two additional optional channels. When configured with six fluorescence channels, the system supports six-target fluorescence detection without cross-interference correction. Compatible probes and dyes include FAM, SYBR Green, VIC, HEX, JOE, TET, TAMRA, Cy3, ROX, Texas Red, CY5, Quasar 705, and Atto 425.
The thermal system provides a temperature control range of 4–99°C, a maximum heating and cooling rate of 4.0°C/s, temperature uniformity of ±0.1°C, and temperature accuracy of ±0.1°C. Its optical detection system uses a maintenance-free LED excitation source and a high-sensitivity photoelectric sensor.
The SLAN®-48P also supports high-resolution melting analysis. The software provides qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, high-resolution melting analysis, and isothermal amplification. Experimental data can be exported in XLSX, CSV, and TXT formats.
Specification
| Product Name | SLAN®-48P Real-Time PCR System |
|---|---|
| Model | SLAN®-48P |
| Sample Capacity | 48 wells |
| Reaction Volume | 15–100 µL |
| Compatible Consumables | 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates |
| Detection Sensitivity | Minimum resolution of 1 copy |
| Linear Range | 100–1010 copies |
| Repeatability | CV <1.00% |
| Sample Linearity | |r| ≥0.9990 |
| Internal Reference Dye Correction | Not required |
| Multiplex Fluorescence Detection | Up to six-target detection without cross-interference correction |
| HRM Analysis | Supported |
| Temperature Control Method | Semiconductor method |
| Temperature Control Modes | Block control and tube control |
| Temperature Range | 4–99°C |
| Maximum Heating and Cooling Rate | 4.0°C/s |
| Temperature Uniformity | ±0.1°C |
| Temperature Accuracy | ±0.1°C |
| Heated Lid | Automatic heated lid |
| Excitation Source | Maintenance-free LED |
| Detector | High-sensitivity photoelectric sensor |
| Data Export Formats | XLSX, CSV, and TXT |
| Custom Reports | Supported |
| Operating System | Microsoft Windows XP, Vista, 7, and 8 |
| Minimum Computer Memory | 4 GB |
| Minimum Hard Drive Capacity | 40 GB |
| Power Input | 220 VAC, 50 Hz |
| Maximum Power | 500 VA |
| Interfaces | RS232 and USB |
| Weight | 16 kg |
Fluorescence Channels
| Channel | Compatible Probes and Dyes | Excitation Wavelength | Detection Wavelength | Configuration |
|---|---|---|---|---|
| Channel 1 | FAM / SYBR Green | 470 nm | 510 nm | Standard |
| Channel 2 | VIC / HEX / JOE / TET / TAMRA / Cy3 | 530 nm | 565 nm | Standard |
| Channel 3 | ROX / Texas Red | 585 nm | 620 nm | Standard |
| Channel 4 | CY5 | 630 nm | 665 nm | Standard |
| Channel 5 | Quasar 705 | 690 nm | 705 nm | Optional |
| Channel 6 | Atto 425 | 420 nm | 470 nm | Optional |
Features
48-Well Reaction Capacity
The SLAN®-48P provides a 48-well reaction platform for real-time fluorescence PCR analysis.
Flexible PCR Consumable Compatibility
The 48-well qPCR instrument accepts 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates. The supported reaction volume is 15–100 µL.
Up to Six Fluorescence Channels
The system provides four standard fluorescence channels. Channel 5 and Channel 6 are optional, allowing the SLAN®-48P to support up to six fluorescence channels.
Six-Target Multiplex Detection
The six-channel configuration supports six-target fluorescence detection without cross-interference correction.
High-Resolution Melting Analysis
The SLAN®-48P supports high-resolution melting curve analysis as part of its software analysis functions.
Precise Temperature Control
The thermal system provides a 4–99°C temperature range, a maximum heating and cooling rate of 4.0°C/s, temperature uniformity of ±0.1°C, and temperature accuracy of ±0.1°C.
Automatic Heated Lid
The SLAN®-48P is equipped with an automatic heated lid.
Maintenance-Free LED Excitation
The optical system uses a maintenance-free LED excitation source and a high-sensitivity photoelectric sensor.
No Internal Reference Dye Correction
The manufacturer states that internal reference dye correction is not required.
Multiple Analysis Modes
The software supports qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, high-resolution melting analysis, and isothermal amplification.
Data Export and Reporting
Experimental data can be exported in XLSX, CSV, and TXT formats. Custom reports are supported.
Application
- Qualitative real-time PCR analysis
- Absolute quantitative PCR analysis
- Relative quantitative PCR analysis
- Standard melting curve analysis
- Endpoint allele genotyping
- Melting-curve genotyping
- High-resolution melting curve analysis
- Isothermal amplification
- Multicolor fluorescence detection
- Multiplex real-time PCR analysis
FAQ
What is the sample capacity of the SLAN®-48P real-time PCR system?
The SLAN®-48P has a sample capacity of 48 wells.
What reaction volume does the SLAN®-48P support?
The supported reaction volume is 15–100 µL.
Which PCR consumables are compatible with the SLAN®-48P?
The system is compatible with 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates.
How many fluorescence channels does the SLAN®-48P support?
The system includes four standard fluorescence channels. Channel 5 for Quasar 705 and Channel 6 for Atto 425 are optional.
Which fluorescent probes and dyes are supported?
The listed probes and dyes are FAM, SYBR Green, VIC, HEX, JOE, TET, TAMRA, Cy3, ROX, Texas Red, CY5, Quasar 705, and Atto 425. Quasar 705 and Atto 425 require the optional fifth and sixth channels.
Does the SLAN®-48P support multiplex real-time PCR?
Yes. The system supports up to six-target fluorescence detection without cross-interference correction when configured with six fluorescence channels.
Does the SLAN®-48P support HRM analysis?
Yes. High-resolution melting curve analysis is included among the listed software analysis functions.
Does the SLAN®-48P require internal reference dye correction?
No. The manufacturer states that internal reference dye correction is not required.
What is the detection sensitivity of the SLAN®-48P?
The stated minimum resolution is 1 copy. The stated linear range is 100–1010 copies, repeatability is CV <1.00%, and sample linearity is |r| ≥0.9990.
What are the temperature performance specifications?
The temperature range is 4–99°C. The maximum heating and cooling rate is 4.0°C/s, temperature uniformity is ±0.1°C, and temperature accuracy is ±0.1°C.
Which analysis functions are available?
The software supports qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, HRM analysis, and isothermal amplification.
Which data export formats are supported?
Experimental data can be exported in XLSX, CSV, and TXT formats. Custom reports are also supported.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
