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$9.00
Sku: P320006-0500
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 20, 2026
Description
RPMI 1640 medium was originally developed at Roswell Park Memorial Institute (RPMI) to culture leukemic cells. RPMI 1640 is the medium code and has since been found widely suitable for a variety of mammalian cells, even including tumor cells, especially for cell culture in suspension. It is now one of the most widely used media. RPMI 1640 medium contains many varieties of amino acids, vitamins and inorganic salts for cell culture. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS) or serum free additives.
Specification
| Full name | RPMI1640 Medium |
Applications
- Cell culture
Components
-
D-Glucose (2.0 g/L)
-
Sodium Bicarbonate (2.0 g/L)
-
Sodium Pyruvate (0.11 g/L)
-
2 mM L-Glutamine
-
25 mM HEPES
-
Phenol Red
Storage
2~8°C
FAQ
What cells can be cultured in RPMI 1640 Medium?
RPMI 1640 Medium is commonly used for culturing PBMCs, lymphocytes, Jurkat cells, THP-1 cells, U937 cells, K562 cells, hybridomas, myeloma cells, and various leukemic cell lines.
Is RPMI 1640 suitable for suspension cell culture?
Yes. RPMI 1640 was originally developed for suspension cultures of human leukemic cells and remains one of the most widely used media for suspension mammalian cell culture.
Does RPMI 1640 require serum supplementation?
Yes. RPMI 1640 contains no proteins, lipids, or growth factors and is typically supplemented with 10% fetal bovine serum (FBS) or other serum-free supplements depending on the cell type.
Does RPMI 1640 require a CO₂ incubator?
Yes. RPMI 1640 contains sodium bicarbonate buffer and is typically used in a 5% CO₂ incubator to maintain physiological pH conditions.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
