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For Research Use Only (RUO)
$1,010.00
Sku: 1201205
Product Description
SHENTEK® Residual Host Cell RNA Sample Preparation Kit is a magnetic particle-based RNA extraction kit for isolation of residual host cell RNA from biological products (e.g., E. coli, 293T). The kit is compatible with manual workflows and automated extraction on the rHCDpurify system. Extracted RNA is suitable for downstream RNA quantitation (real-time qPCR) and other nucleic acid-based assays. The kit includes reagents for protein digestion (Proteinase K), binding, washing, magnetic particles, RNase inhibitor, glycogen carrier, and elution reagents. The kit is intended for research use only.
Technical Specifications
| Parameter | Details |
|---|---|
| Intended use | Extraction of residual host cell RNA from biological samples for downstream RNA quantitation (qPCR) |
| Kit capacity | Reagents for 100 extractions |
| Extraction method | Magnetic particle-based RNA binding, wash and elution |
| Proteinase K digestion | Typical: add 100 µL sample + 110 µL Proteinase K digestion solution; incubate 55 °C for 60 min; machine max digestion volume 300 µL |
| Proteinase K dosing guidance | For sample protein concentration 0–100 mg/mL: 10 µL Proteinase K per sample; for 100–200 mg/mL: 20 µL Proteinase K per sample (volumes mixed with Proteinase K Buffer as tabled) |
| Binding | Per sample: add 10 µL 5M NaCl + 220 µL working binding solution; add 200–400 µL isopropanol (≈1/3–1/2 total volume); add 10–15 µL magnetic particles; mix 3–5 min |
| Washing | Wash buffer A: 700 µL; Wash buffer B: 700 µL; air-dry magnetic particles 30 s–3 min to remove residual ethanol |
| Elution | Add 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v); incubate 50 °C for 5 min with intermittent vortexing; separate beads and recover eluate |
| Magnetic particles | Use 10–15 µL per sample; vortex to re-suspend before use; recommended storage 2–8 °C |
| Working binding prep | Per sample working binding: 200 µL Binding solution + 10 µL diluted Precipitation solution I (1:99 in Dilution solution) + 10 µL Glycogen |
| pH requirement | Ideal sample pH 6.0–8.0; adjust with 1 M HCl or 1 M NaOH if pH <5.0 or >9.0 |
| Storage stability | Kit components stored at indicated temperatures are stable for up to 24 months (check label expiration date) |
| Compatibility / equipment | Compatible with rHCDpurify automated system and manual extraction; requires real-time PCR system for downstream quantitation |
Features
- Magnetic particle-based separation: Efficient magnetic bead capture of RNA for high recovery and clean eluates suitable for qPCR
- Automated and manual compatibility: Validated for use with rHCDpurify instrument and a manual workflow
- Complete kit for RNA extraction: Includes Proteinase K digestion reagents, binding/wash/elution buffers, magnetic particles, RNase inhibitor and glycogen carrier
- Designed for residual host cell RNA: Optimized procedure for extraction of residual RNA from biological products and plasmid preparations
- Stable shelf life: Components stored at proper temperatures are stable up to 24 months
Applications
- Extraction of residual host cell RNA from biopharmaceutical samples (e.g., E. coli, 293T)
- Sample preparation for RNA quantitation by real-time qPCR
- Preparation of plasmid DNA samples with residual host RNA assessment
- Routine QC workflows in research and development laboratories
Kit Contents
Proteinase K Buffer (NND026) 10 mL × 1Room temperature
Binding solution (NND016) 20 mL × 1Room temperature
Wash buffer A (NND014) 30 mL × 1 (requires addition of 40 mL ethanol before first use)Room temperature
Elution solution (NND061) 10 mL × 1Room temperature
Dilution solution (NND062) 12 mL × 2Room temperature
Magnetic particles (NND030) 750 µL × 2 tubes2-8 °C
5M NaCl (NND040) 500 µL × 2 tubes2-8 °C
Proteinase K (NND023) 500 µL × 2 tubes-20 °C
RNase inhibitor (NND060) 50 µL × 1 tube-20 °C
Glycogen (NND035) 500 µL × 2 tubes-20 °C
Precipitation solution I (NND003) 25 µL × 1 tube-20 °C
* Total: 100 extractions | Method: Magnetic particle-based RNA extraction (manual or rHCDpurify automated)
Attention
• For Research Use Only. Read Material Safety Data Sheets (MSDS) and follow handling instructions.
• Wear appropriate PPE: gloves, protective eyewear, mask and lab clothing.
• Add 40 mL ethanol to Wash buffer A before first use; prepare 70% ethanol Wash buffer B from Dilution solution.
• Prepare working elution solution by mixing RNase inhibitor : Elution solution = 1 : 200 (v:v) in RNase-free area.
• Prepare diluted Precipitation solution I at 1:99 (Precipitation solution I : Dilution solution) before preparing working binding solution.
• Ideal sample pH is 6.0–8.0. Adjust samples to neutral pH if outside range.
• Do not over-dry magnetic particles; over-drying may reduce elution efficiency.
• Perform downstream assays the same day as extraction to ensure accurate results.
• When removing supernatant, do not disturb or aspirate magnetic bead pellet; use low-retention tips and centrifuge as instructed.
• UV sterilize rHCDpurify instrument interior (suggested 15 minutes) before runs; allow >30 min between extractions if required.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What downstream assays are compatible with the extracted RNA?
Extracted RNA is suitable for RNA quantitation by real-time qPCR and other nucleic-acid based assays. Use appropriate DNase treatment if required by your assay.
Q2: How do I prepare Wash buffer A and B before first use?
Add 40 mL of ethanol to Wash buffer A (NND014) before first use. Prepare Wash buffer B by mixing Dilution solution (NND062) with ethanol to make 70% ethanol.
Q3: What are the recommended elution volumes and conditions?
Elute with 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v). Incubate at 50 °C for 5 minutes with intermittent vortexing, then separate beads and recover eluate.
Q4: Can this kit be used on an automated platform?
Yes. The kit is compatible with the rHCDpurify automated extraction system (program rHCR-1201205) as well as manual extraction workflows.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
