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Introduction
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| qPCR Reaction Buffer | -20℃, protect from light | 4x100 Reactions | SHENTEK™ | Probe-based qPCR |
| HEK293 Primer&Probe MIX-75 | ||||
| HEK293 Primer&Probe MIX-122 | ||||
| HEK293 Primer&Probe MIX-244 | ||||
| HEK293 Primer&Probe MIX-562 | ||||
| IPC MIX | ||||
| DNA Dilution Buffer (DDB) | -20℃ | |||
| Vero DNA Control |
Overview
SHENTEK™ Residual HEK293 DNA Size Analysis Kit is used to quantitate HEK293 DNA residues of different fragment sizes for various stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes real-time PCR technology to perform rapid and specific quantitation of residual HEK293 DNA fragments in samples. It is designed to amplify four different fragments (75bp, 122bp, 244bp, 562bp) for the accurate determination of their size distribution with the LOD level of femtograms. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Specification
| Range | 3x10-2-3x102 pg/μL, R2 ≥0.990, AE=96.8%-102.1% |
| Accuracy | Recovery = 76.0%-121.1%, CV<40% |
| LOQ | 0.03 pg/μL |
| Precision | 4.0%-35.4% (<40%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments |
Related Products
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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