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Description
SHENTEK® Residual E1A DNA Quantitation Kit is used to quantitate residual E1A DNA from host cell, such as HEK293 and 293T cells, in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique to perform rapid, specific, and reliable quantitation assay with residual E1A DNA. IPC (Internal Positive Control) is included in the kit to evaluate the performance of each PCR reaction. The kit provides E1A linear DNA Control and E1A non-linear DNA Control for customers to choose on their own needs. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Overview
| Components | Shipping Condition | Unit Size | Detection Method |
| E1A linear DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| E1A non-linear DNA Control | -20℃ | ||
| qPCR Reaction Buffer | -20℃, protect from light | ||
| E1A Primer&Probe MIX | -20℃, protect from light | ||
| IPC MIX | -20℃, protect from light | ||
| DNA Dilution Buffer (DDB) | -20℃ |
Specification
| Range | 4.97x101-4.97x106 pg/μL, R2=0.99980, E=93.8% |
| Accuracy | Recovery = 77.8%-116.4%, CV<30% |
| LOQ | 16.6 copies/μL |
| LOD | 0.994 copies/μL |
| Precision | 2.3%-14.5% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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