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$235.00
Sku: GMP-TL651-0100
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
The recombinant human Vitronectin (VTN) protein is a high-purity multifunctional glycoprotein transiently expressed in HEK-293 cells, featuring a C-terminal polyhistidine tag for precise purification via nickel-affinity chromatography.
As a core component of the extracellular matrix (ECM), vitronectin mediates cell adhesion, spreading, and migration through RGD sequence-specific binding to integrin receptors (αvβ3, αvβ5, αvβ1, etc.), while regulating stem cell proliferation and differentiation.
In cell and gene therapy applications, this protein serves as a critical element in serum-free culture systems: its multimeric form efficiently incorporates into biomimetic scaffolds, providing in vivo-like adhesion signals for mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs) to significantly enhance ex vivo expansion efficiency and stemness maintenance.
Furthermore, it synergizes with growth factors (e.g., TGF-β) to optimize microenvironments for organoid development and tissue-engineered grafts, promoting vascularized tissue regeneration.
The native conformation ensures its full bioactivity.
Specifications
| Parameter | Specification | Results |
|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particle | Should be no visible particle | Compliance |
| Moisture | ≤4.0% | 2.29% |
| pH | 6.5~7.5 | 7.01 |
| Purity | >95.0% | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | By measuring its ability to promote hiPS cell adhesion, this effect ED50 < 2.4 μg/mL | 1.139 μg/mL |
Features
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✔
HEK293-Expressed His-Tagged Vitronectin
Recombinant human vitronectin (Asp19–Leu478) expressed in HEK293 cells with a C-terminal 6×His-tag, predicted molecular weight 53 kDa.
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✔
High Purity and Quality Compliance
Purity >95.0% with electrophoretic identity consistent with the expected molecular weight; no visible particles; moisture 2.29%; pH 7.01 within 6.5–7.5 specification.
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✔
Low Endotoxin and Sterility Assurance
Endotoxin level <0.1 EU/µg (LAL method), mycoplasma negative, and sterility confirmed with no growth.
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✔
Validated hiPS Cell Adhesion Activity
Biological activity determined by promotion of hiPS cell adhesion with ED₅₀ ≤2.4 µg/mL (measured 1.139 µg/mL).
Applications
hiPS Cell Adhesion Support
Promotes adhesion of human induced pluripotent stem (hiPS) cells for in vitro culture applications.
Serum-Free Stem Cell Culture
Functions as a defined extracellular matrix component supporting MSC and iPSC expansion and stemness maintenance.
Cell Culture Surface Coating
Used for coating culture plates to enhance cell attachment via integrin-mediated interactions.
Organoid and Tissue Engineering
Supports microenvironment optimization in organoid development and tissue-engineered graft studies.
Data Figures
Stability & Storage
- Lyophilized powder: Stable for 24 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: For lyophilized powder, dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥100 µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
