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$45.00
Sku: Y04401-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
TNF-α (Tumor Necrosis Factor-α) is an important cytokine primarily produced by activated immune cells such as macrophages, lymphocytes, and NK cells. It plays a central role in physiological and pathological processes including inflammation, immune regulation, and apoptosis. As a pro-inflammatory cytokine, TNF-α participates in normal inflammatory and immune responses. It orchestrates tissue homeostasis by coordinately regulating the production of other cytokines, as well as modulating cell survival and death. TNF-α exerts diverse functions in various developmental and immune processes—such as inflammation, differentiation, lipid metabolism, and apoptosis—and is implicated in numerous diseases.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y04401 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. |
| Accession | P01375 |
| Alternative Names | umor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2 |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 17.4 kDa |
| Expression Region | Val77-Leu233 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Modulates hematopoietic progenitor apoptosis and differentiation under stress.
Immunology
Key pro-inflammatory cytokine, regulates immune responses.
Stem Cell Research
Used to mimic inflammatory microenvironments affecting stem cell fate.
Cell Biology
Regulates apoptosis, proliferation, and inflammation signaling.
Data Figures
Figure 1: Recombinant Human TNFα (Cat. No. Y04401) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04401 SDS-PAGE.
Figure 2: Recombinant Human TNFα (Cat. No. Y04401) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. The bioactivity of Y04401 was equivalent to other competing products.
Figure 3: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
