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$104.00
Sku: TL-303-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
The recombinant human Tumor Necrosis Factor Alpha (TNF-α) protein is a high-purity homotrimeric cytokine produced in HEK-293 expression system via transient transfection, featuring a C-terminal polyhistidine tag for nickel-affinity chromatography purification.
As the prototypic member of the TNF superfamily, TNF-α is produced primarily by macrophages and exerts its broad spectrum of pleiotropic biological effects by binding to its receptors TNF-R1 (CD120a/p55) and TNF-R2 (CD120b/p75).
It plays a central role in regulating immune cell activity, capable of inducing apoptotic cell death in certain tumor cell lines, triggering inflammation, inhibiting viral replication, and critically modulating processes such as cell proliferation, differentiation, lipid metabolism, and coagulation.
However, dysregulation of TNF-α is implicated in a variety of human diseases, including cancer (exhibiting dual roles in both antitumorigenesis and tumor promotion/metastasis), septic shock, fever, autoimmune diseases (such as rheumatoid arthritis and Crohn’s disease), graft-versus-host disease, Alzheimer’s disease, and major depression.
Recombinant TNF-α is utilized clinically as an immunostimulant under the INN tasonermin.
The native homotrimeric configuration preserves full bioactivity, while the His-tag design enables precise purification.
Specifications
| Product | Parameter | Specification | Results |
|---|---|---|---|
| TL-303-0010 | Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance | |
| Visible Particle | Should be no visible particle | Compliance | |
| Moisture | ≤4.0% | 1.0% | |
| pH | 6.5~7.5 | 7.2 | |
| Purity | >95.0% | Compliance | |
| Endotoxin | <0.1EU/μg | <0.1EU/μg | |
| Mycoplasma | Should be negative | Negative | |
| Sterility | No growth | No growth | |
| Biological Activity | In the presence of metabolic inhibitor actinomycin D, cytotoxicity assay was performed on L929 cells, with specific activity ≥ 1.0 × 107 IU/mg | 4.27 × 106 IU/mg | |
| GMP-TL303-0100 | Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance | |
| Visible Particle | Should be no visible particle | Compliance | |
| Moisture | ≤4.0% | 0.6% | |
| pH | 6.5~7.5 | 7.3 | |
| Purity | >95.0% | Compliance | |
| Endotoxin | <0.1EU/μg | <0.1EU/μg | |
| Mycoplasma | Should be negative | Negative | |
| Sterility | No growth | No growth | |
| Biological Activity | In the presence of metabolic inhibitor actinomycin D, cytotoxicity assay was performed on L929 cells, with specific activity ≥ 1.0 × 107 IU/mg | 7.03 × 107 IU/mg |
Features
-
✔ Native Homotrimeric TNF-α Activity
Homotrimeric configuration preserves full bioactivity for TNF-R1 (CD120a/p55) and TNF-R2 (CD120b/p75) engagement in downstream signaling studies.
-
✔ HEK-293 Expression with His-Tag Purification
Produced via transient transfection in HEK-293 cells with a C-terminal polyhistidine tag for nickel-affinity chromatography purification.
-
✔ Stringent QC for Cell-Based Assays
Lots meet appearance/identity/visible particle criteria, with endotoxin <0.1 EU/μg, mycoplasma negative, and sterility showing no growth.
-
✔ Validated L929 Cytotoxicity Bioassay
Bioactivity measured by L929 cell cytotoxicity assay in the presence of actinomycin D, with lot results reported for TL-303-0010 and GMP-TL303-0100.
Applications
TNFR Signaling Studies
Supports mechanistic studies of TNF-R1/TNF-R2-mediated immune signaling and downstream pathway activation.
L929 Cytotoxicity Assays
Used in cytotoxicity assay workflows on L929 cells in the presence of actinomycin D for functional activity evaluation.
Inflammation Research Models
Applicable for studying inflammatory mediator induction and immune-cell activity regulation in vitro.
Cancer and Immunology Studies
Supports research on TNF-α roles in tumor cell apoptosis and broader immunology-related investigations.
Data Figures
Stability & Storage
- Lyophilized powder: Store at -20°C or below to maintain product stability and activity.
- Reconstitution: Not provided.
- Handling: Avoid repeated freeze-thaw cycles to prevent protein degradation.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
