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$95.00
Sku: GMP-TL906-0100
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by April 30, 2026
Description
Activated T cells generally cannot survive for a long time in vitro culture, and the addition of IL-2 can promote their long-term and sustained proliferation.
The surface of stationary T cells does not express IL-2R and does not respond to IL-2. T cells activated by mitogen or other stimuli can express IL-2R and become target cells of IL-2. IL-2 can also induce increased IL-2R expression in target cells.
The expression of IL-2R on T cells is transient, generally reaching its peak 2–3 days after activation and disappearing around 6–10 days. With the disappearance of IL-2R, T cells lose their ability to respond to IL-2.
Therefore, in order to maintain the long-term growth of normal T cells in vitro, it is necessary to constantly stimulate T cells with mitogen or other stimulators to maintain the expression of IL-2R.
NK cells are the only lymphoid cells that normally express IL-2R and therefore always maintain reactivity to IL-2. However, only the IL-2R β chain and γ chains are expressed on stationary NK cells, which have a low affinity for IL-2 and can only react with high concentrations of IL-2.
Once NK cells are activated, they express the IL-2R α chain, becoming a high-affinity receptor.
Specifications
| Cytokines Details | |
|---|---|
| Expression Host | E. coli |
| Synonyms | TCGF, Lymphokine, Interleukin |
| Purity | > 90% as determined by SDS-PAGE |
| Biological Activity | Determined by the dose-dependent stimulation of CTLL-2 cell proliferation, corresponding to an activity of ≥ 1 × 10⁷ U/mg |
| Endotoxin | < 0.1 EU per 1 μg of protein, determined by the LAL method |
| Molecular Mass | The recombinant human IL-2 protein has a predicted molecular mass of 15.4 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Typically supplemented with 6% mannitol as a stabilizing protectant prior to lyophilization |
Features
- ✔ High-Potency IL-2: ≥1 × 10⁷ U/mg bioactivity ensures strong and reproducible proliferation of activated T and NK cells.
- ✔ Tag-Free Design: Eliminates Fc-mediated off-target effects, supporting clean signaling in immune cell culture.
- ✔ Ultra-Low Endotoxin: <0.1 EU/µg endotoxin, suitable for sensitive cell cultures and therapy process development.
- ✔ Optimized Growth: Effectively sustains proliferation during the IL-2R expression window following activation.
- ✔ Stable Formulation: Mannitol-protected lyophilized format ensures excellent stability and reliable performance.
Applications
CAR-T/TCR-T Expansion
Supports controlled and scalable expansion of genetically modified T cells during ex vivo manufacturing.
NK Cell Activation
Promotes NK cell proliferation and enhances cytotoxic activity and cytokine secretion.
TIL & CIK Culture
Enables sustained growth of tumor-reactive and cytokine-induced killer cells in vitro.
Signaling Studies
Used to study IL-2/IL-2R signaling, receptor dynamics, and lymphocyte proliferation mechanisms.
Data Figures
Figure 1: Recombinant Human IL-2 Protein Analysis
Stability & Storage
- Lyophilized preparation can be stored at 2-8 ℃.
- 6 months at -20℃ under sterile conditions after reconstitution.
- 12 months at -80℃ under sterile conditions after reconstitution.
- Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
- Avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
