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$260.00
Sku: GMP-TL902-0050
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 11, 2026
Description
IL-18 is a pleiotropic proinflammatory cytokine belonging to the IL-1 superfamily, which requires caspase-1-mediated proteolytic activation to engage the heterodimeric IL-18Rα/β receptor, triggering NF-κB and MAPK signaling pathways that drive IFN-γ production in Th1 cells, NK cells, and macrophages.
It synergizes with IL-12 to enhance IgG2a class switching while suppressing IL-4-dependent IgE/IgG1 production.
In cell therapy manufacturing, the recombinant IL-18 protein serves as a critical culture component to augment CAR-T/NK cell effector functions and persistence through coordinated action with IL-15/IL-12, while potentiating FasL-mediated cytotoxicity and promoting memory T-cell differentiation, thereby optimizing the functional maturity and therapeutic potency of antitumor immune cell products.
Its activity is tightly regulated by IL-18 binding protein (IL-18BP), establishing a dynamic equilibrium that offers targeted therapeutic strategies for inflammatory and allergic disorders.
Specifications
| Cytokines Details | |
|---|---|
| Synonyms | Interleukin-18, Iboctadekin, Interleukin-1 gamma, IFN-gamma-inducing factor |
| Accession | UniProt Q14116 |
| Amino Acid Sequence | Tyr37–Asp193 |
| Tag | Tag-Free |
| Expression System | E. coli |
| Predicted Molecular Weight | 18.2 kDa |
| Purity | > 95% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Induces IFN-γ secretion in KG-1 cells with TNF-α co-stimulation, corresponding to a specific activity of ≥ 2.0 × 106 IU/mg |
| Formulation | Lyophilized from sterile 20 mM phosphate-buffered saline (PBS), pH 7.4, typically containing 6–8% (w/v) mannitol as a stabilizing protectant |
Features
-
✔
Potent IFN-γ Induction
Strongly activates NF-κB/MAPK signaling to drive IFN-γ production in Th1 and NK cells.
-
✔
Synergistic Immuno-Activation
Exhibits powerful synergy with IL-12 and IL-15 to amplify cytotoxic immune responses.
-
✔
High Biological Activity
Validated by KG-1 cell IFN-γ secretion assay, ensuring reliable functional potency.
-
✔
Tag-Free Native Cytokine
Preserves authentic IL-18 structure and signaling without fusion-induced bias.
Applications
CAR-T and NK Cell Functional Enhancement
Boosts effector function, cytotoxicity, and antitumor activity during cell manufacturing.
IFN-γ–Driven Immune Activation Studies
Ideal for Th1 polarization and inflammatory signaling research.
Combination Cytokine Cell Culture Systems
Used with IL-12 / IL-15 to optimize immune cell maturation and persistence.
Immuno-Oncology Research
Supports development of highly potent immune effector cell therapies.
Stability & Storage
- √ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored inthe original sealed container under desiccant.
- √ Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4)maintaining final concentration ≥100 µg/mL to prevent adsorption.
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
