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Phase Lock Gel Alternative
$349.00
Sku: M2302-20-H
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
PhaseShield™ Gel Tubes are preloaded phase separation tubes designed to improve the safety, efficiency, and reproducibility of phenol–chloroform and chloroform-based nucleic acid extraction workflows. Acting as a high-performance alternative to traditional Phase Lock Gel, the specialized gel forms a stable physical barrier between the aqueous and organic phases during centrifugation, driven by density differences.
This clear phase separation effectively prevents phenol or chloroform carryover into the aqueous layer, enabling clean recovery of DNA or RNA without interphase contamination. By eliminating manual phase separation and minimizing handling errors, PhaseShield™ Gel Tubes help increase nucleic acid recovery yields by up to 30%, while significantly reducing operator exposure to hazardous organic solvents.
Available in Heavy and Light density grades, PhaseShield™ Gel Tubes are compatible with a wide range of organic extraction systems and standard centrifuges.
PhaseShield™ Extraction Workflow
Specification
| Specification | Gel Density Grade |
|---|---|
| Options | Heavy / Light |
Selection Guide
| Aqueous phase | Organic phase | |||
|---|---|---|---|---|
| Phenol/chloroform/ isoamyl alcohol (25:24:1) |
Chloroform/isoamyl alcohol (24:1) |
Water/buffers sat. chloroform (24:1) |
Water or buffers saturated phenol |
|
| <0.5 M NaCl | H, L | H, L | H, L | L |
| <1 mg/ml BSA | H, L | H, L | H, L | L |
| Plasmid DNA extraction | H | H | H | X |
| Tissue homogenate DNA | H, L | H, L | H, L | L |
| RNA extraction | H | H | H | L |
Density Guidelines: Heavy (H) is ideal for plasmids, RNA, and viscous samples with high impurities. Light (L) is suitable for restriction digestion, cDNA synthesis, and conventional tissue DNA extraction.
Features
- Stable Phase Separation Barrier: Forms a consistent gel layer between phases.
- Reduced Organic Carryover: Prevents phenol/chloroform contamination.
- Higher Recovery: Enables complete aqueous phase collection without loss.
- Laboratory Safety: Minimizes operator exposure to hazardous solvents.
Applications
- Phenol-Based RNA Extraction: Total RNA isolation using phenol or phenol-chloroform methods (e.g., TRIzol®-based workflows)
- RNA Purification from Complex Samples: Extraction of RNA from tissues, cells, and other biologically complex matrices
- Phase Separation and Cleanup: Efficient separation of the aqueous RNA phase from organic solvents during centrifugation
- Viscous or High-Impurity Samples: RNA extraction from tissue homogenates and cell lysates with high protein or lipid content
- Routine Molecular Biology Workflows: RNA preparation for downstream RT-PCR, qPCR, cDNA synthesis, and gene expression analysis
Ordering Information
| Product: PhaseShield™ Gel Tubes (2ml) |
| Quantity: 200 Tubes per pack |
| Default Grade: Heavy / Light |
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
