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Sku: 1509841
Categories: KitQC Kits


Components Shipping Condition Unit Size Product Line Detection Method
MyqPCR reaction buffer (2G) -20℃, protect from light 50 Reactions MycoSHENTEK Probe-based qPCR
MyPrimer & Probe MIX (2G)      
Internal control (IC) (2G)* -20℃      
Positive control (PC) (2G)*      
DNA dilution buffer      



MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G) is used, together with MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G), to qualitatively determine whether there is mycoplasma, spiroplasma, or acholeplasma contamination in master cell bank, working cell bank, virus seed stock, or cell culture-derived products, etc. The integrated system has been fully validated per European Pharmacopoeia Chapter 2.6.7 and Japanese Pharmacopoeia XVII, at a detection limit of 10 CFU/mL.
The detection kit uses real-time PCR technology to detect more than 200 species of Mycoplasma, Spiroplasma and Acholeplasma with high specificity. It has been validated in a variety of matrices, as well as on non-mycoplasma species, production cells, and engineered bacteria and fungi.



Inclusivity More than 180 species of Mycoplasma, Spiroplasma and Acholeplasma
Detection Limit 10 CFU/mL or 1 genome copy/μL (Positive cut-off: 95%)
Specificity No matrix-induced effect across different sample matrices and no cross-reactivity to nearly 30 types of cell lines
Robustness Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments
Consistent LOC in different matrices


Related Products

MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G)


User Guide

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.


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