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Introduction
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| MB qPCR Reaction Buffer | -20℃, protect from light | 50 Reactions | MycoSHENTEK | Probe-based qPCR |
| MB Primer & Probe MIX | ||||
| MB Internal Control (IC) | -20℃ | |||
| MB Positive Control (PC) | ||||
| DNA Dilution Buffer (DDB) |
Overview
MycoSHENTEK™ Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK™ Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, etc., using real-time qPCR techonology. The integrated system has been fully validated at a detection limit of 10-100 CFU/mL to detect more than 100 different Mycobacteria species. The system contains dUTP, and if used with the UNG enzyme to prevent contamination and eliminate carry-over contamination Internal Control (IC) can be added to a PCR amplification reaction mixture to evaluate whether a test sample inhibits the amplification reaction and may result in false negative results. It can also be added to the sample before extraction to evaluate the extraction efficiency. For extraction information, please refer to MycoSHENTEK™ Mycobacteria DNA Extraction Kit User Guide (Product No.: 1503601).
Specification
| Inclusivity | More than 100 different Mycobacteria species |
| Detection Limit | 10 CFU/test or 20 copies/test (Positive cut-off: 95%) |
| Specificity | No matrix-induced effect across different sample matrices and no cross-reactivity to different cell lines, bacteria or fungi |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Roche, Jena, HZSKBio qPCR equipments | |
| Consistent performance to the slight variations of reaction system and amplification procedure |
Related Products
MycoSHENTEK™ Mycobacteria DNA Extraction Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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