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Mouse IL2; mIL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
$198.00
Sku: RP570-10UG
Categories:
Activity Collection
Bcakground
Interleukin-2 (IL-2) is an O-glycosylated four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells, and eosinophils (1–3). Mature mouse IL-2 shares 56% and 73% amino acid (aa) sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch (4). Mouse and human IL-2 exhibit cross-species activity (5).
The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (6–8). The 55 kDa IL-2Rα is specific for IL-2 and binds with low affinity. The 75 kDa IL-2Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common γ chain (γc/IL-2Rγ), which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2Rβ and γc.
IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2Rα synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes (9). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17-polarized cells (10–12). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).
Reference
1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
3. Kashima, N. et al. (1985) Nature 313:402.
4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
7. Wang, X. et al. (2005) Science 310:1159.
8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
11. Laurence, A. et al. (2007) Immunity 26:371.
12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Specifications
| Synonyms | Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2;T-cell growth factor; TCGF |
| Accession # | P04351 |
| Source | Human embryonic kidney cell, HEK293-derived mouse IL-2 protein |
| Ala21-Gln169 | |
| Predicted Moleucular weight | 17.2kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening | |
| Storage and Stability | Avoid repeated freeze-thaw cycles |
| It is recommended that the protein be aliquoted for optimal storage | |
| 12 months from date of receipt, -20 to -70 °C as supplied | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method |
| Activity | Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells |
| The EC50 for this effect is 0.01-0.1 ng/mL |
SDS-PAGE
Bioactivity
Documents
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