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Human CSF-1 Protein, Human M-CSF Protein, Human MCSF Protein
$198.00
Sku: RP400-10UG
Categories:
Activity Collection
Bcakground
Macrophage Colony Stimulating Factor(M-CSF) , also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion (10）
Reference
1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
2.Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
4. Ryan, G.R. et al. (2001) Blood 98:74.
5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
6. Nandi, S. et al. (2006) Blood 107:786.
7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
Specifications
| Synonyms | Colony stimulating factor 1 (macrophage); CSF1; CSF-1; macrophage colony-stimulating factor 1; MCSF |
| Accession # | P09603 |
| Source | Human embryonic kidney cell, HEK293-derived human M-CSF/CSF1 protein |
| Glu33-Ser190 | |
| Predicted Moleucular weight | 22.0 kDa(Monomer) |
| Form/Structure | Dimer in solution |
Components and Storage
| Formulation | Solution protein. |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method |
| Activity | Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. |
| The EC50 for this effect is 0.2-1.5 ng/mL. |
SDS-PAGE
Bioactivity
Documents
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