Human IFN-γ ELISA Kit SHENTEK®

Description

SHENTEK® Human IFN-γ ELISA Kit is suitable for the quantitation of human interferon-gamma (IFN-γ), for example, in potency assays of CAR-T cells.

Overview

This kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA) using a double-antibody sandwich technique to detect human IFN-γ. An antibody specific to human IFN-γ is employed in the assay to capture any IFN-γ present in the sample. Both the calibration standards and test samples are simultaneously added to a microtiter plate coated with an affinity-purified capture antibody, followed by incubation and washing. A biotinylated antibody is then added to bind human IFN-γ, which subsequently reacts with streptavidin-labeled HRP (horseradish peroxidase). TMB (3,3′,5,5′-tetramethylbenzidine) substrate is introduced into the reaction, and HRP catalyzes the oxidation of TMB by H₂O₂ to produce a blue-colored product (maximum absorption peak at 655 nm). The stop solution is then added to terminate the enzymatic reaction, resulting in a yellow-colored product (maximum absorption peak at 450 nm). The absorbance at 450 nm is positively correlated with the human IFN-γ concentration in both the calibration standards and the samples. The concentration of human IFN-γ in the samples can be calculated using a dose–response curve.

Introduction

Specification