0 item(s)
View cart
You have no items in your shopping cart.
Product Description
This is a sandwich ELISA kit for quantitative detection of Human Interferon Gamma (IFN-γ). Microtiter strips are pre-coated with an affinity-purified anti-Human IFN-γ capture antibody. Samples and calibration standards are incubated in the wells, followed by binding of a biotinylated detection antibody and detection with streptavidin–HRP. TMB substrate is oxidized by HRP to produce a blue product (max absorbance ~655 nm) that is converted to yellow upon addition of stop solution (read at 450 nm). The assay is calibrated against recombinant human IFN-γ (NIBSC/WHO reference evaluated) and is intended for research use (e.g., potency assays for CAR-T cells and measurement in cell culture supernatants).
Technical Specifications
| Parameter | Details |
|---|---|
| Package | 96 tests/Kit |
| Intended use | Quantitation of Human IFN-γ in research applications (e.g., CAR-T potency assays). Not for clinical use. |
| Linearity & Range | 5 - 400 pg/mL, R2 ≥ 0.990 |
| LLOQ | 5 pg/mL |
| Typical calibration curve (4-PL) | 4-PL parameters: A = 8.44302; B = -1.01023; C = 1192.73656; D = 0.00183; R2 = 0.99994 |
| Specificity / Cross-reactivity | Recognizes natural and recombinant human IFN-γ. No cross-reactivity with recombinant human IL-2, IL-6, IL-7, IL-10, IL-12, IFN-β, IFN-α2b, IFN-γ R1, IFN-γ R2; no cross-reactivity with rat IFN-γ or mouse IFN-γ. |
| Calibration | Calibrated against recombinant human IFN-γ. NIBSC/WHO Human IFN-γ International Standard 82/587 evaluated. Conversion: NIBSC (82/587) (IU/mL) ≈ 0.0065 × Human IFN-γ (pg/mL). |
| Sample types | Cell culture supernatants and other clear biological samples (insoluble material must be removed by centrifugation/filtration). |
| Sample pH requirement | pH 6.0–8.5 |
Features
- Sandwich ELISA format: Double-antibody sandwich technique for specific capture and detection of IFN-γ.
- High sensitivity: Low limit of quantitation (LLOQ) of 5 pg/mL and linear range 5–400 pg/mL.
- Validated calibration: Calibrated to recombinant human IFN-γ and evaluated against NIBSC/WHO standard.
- Pre-coated ready-to-use strips: Microtiter strips pre-coated with capture antibody; supplied in sealed bag with desiccant.
- Minimal cross-reactivity: No detectable cross-reactivity with listed human cytokines or rodent IFN-γ tested.
- Research application focus: Suitable for CAR‑T cell potency assays and cytokine measurement in research settings.
Applications
- Quantitation of human IFN-γ in research samples
- Potency assays for CAR‑T cells
- Measurement of IFN-γ in cell culture supernatants
- Preclinical research and in vitro cytokine profiling
Kit Contents
Human IFN-γ Calibration Standard (lyophilized)Reconstitute with Reconstitution Solution (300 μL); ST0 can be stabilized at -65 °C for 15 days; avoid repeated freeze–thaw; dissolved standards for single use
Anti-Human IFN-γ Microtiter Strips (8 wells × 12 strips)Store sealed with desiccant at 2-8 °C; after opening store in bag with desiccant at 2-8 °C (up to 90 days)
Reconstitution Solution (1.5 mL)2-8 °C (used to dissolve Calibration Standard)
Diluent (2 × 25 mL)2-8 °C
Wash Buffer Concentrate (10×) (2 × 25 mL)2-8 °C; dilute 1:10 with ultra-pure water before use
Anti-Human IFN-γ: Biotinylated Conjugate (100×) (120 μL)2-8 °C; protect from light; dilute 1:100 with Diluent immediately before use; recommended to centrifuge before use
Streptavidin-HRP (100×) (140 μL)2-8 °C; protect from light; dilute 1:100 with Diluent immediately before use; recommended to centrifuge before use
TMB Substrate (12 mL)2-8 °C; protect from light; equilibrate to RT 20 min before use; should be colorless
Stop Solution (1 M HCl, 6 mL)2-8 °C; corrosive—avoid contact with skin/eyes; wear goggles
Sealing Film (3 pieces)Room temperature; use to cover plate during incubations to prevent evaporation
* Total: 96 tests/Kit | Method: ELISA (sandwich)
Attention
• For Research Use Only (not for clinical use).
• Store kit at 2-8 °C. Check expiration dates on labels.
• Avoid repeated freeze–thaw cycles of standards and samples; long‑term storage at -65 °C or below recommended for samples.
• Dissolved Calibration Standards, 1× Biotinylated Conjugate and 1× Streptavidin-HRP are recommended for single use (prepare freshly).
• Do not mix reagents from different lot numbers.
• Use fresh sterile or ultra‑pure water; avoid water >37 °C.
• Avoid samples containing sodium azide (NaN3) which will inhibit HRP and cause underestimation.
• Stop Solution is 1 M HCl — avoid direct contact with eyes, skin and clothing; wear goggles and protective equipment.
• TMB Substrate should be colorless before use; discard if colored.
• Seal microplate during incubations to prevent evaporation; avoid drying wells before substrate addition.
• Centrifuge conjugate reagents (100×) before dilution to avoid loss of reagent.
• Minimum recommended pipetting volume for dilution accuracy: 5 μL.
• Complete plate reading within 30 minutes after termination.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What is the assay linear range and sensitivity?
Linear range is 5–400 pg/mL. LLOQ is 5 pg/mL.
Q2: What sample types can be measured with this kit?
Cell culture supernatants and other clear biological samples after removal of particulates by centrifugation or filtration. Validate sample dilutions prior to routine use.
Q3: How should I store the kit and reagents?
Store the kit at 2–8 °C. Keep microtiter strips sealed with desiccant at 2–8 °C (opened strips stable up to 90 days). Reconstituted standards and freshly diluted conjugates are for single use.
Q4: What should I do if my substrate is colored or background is high?
If TMB substrate is colored, discard and contact technical support. For high background, review washing steps, avoid cross-contamination, clean equipment and prepare fresh reagents.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
