Human IFN-γ Precoated ELISA kit-96T-BioSci™

Product Description

The human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by "sandwich one step". The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.

Product Details

Detection range : 12.5-400 pg/mL

Sensitivity: 5 pg/mL

Precision : Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%

Stored : 2 - 8 °C

Verified Reactivity：Human

Reagents Provided and Reconstitution

Material Required but not Provided

• 96-well ELISA plate (microplate spectrophotometer).

• Pipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.

• Distilled water, fresh absorbent paper.

• Vortex mixer and magnetic stirrer

Scientific Data

Assay Procedure Summary

• Add 100 μL of sample, standard or control per well

• Add 50 μL of diluted Biotinylated Detection antibody to all wells

• Incubate 2 hours at room temperature

• Wash three times

• Add 100 μL of streptavidin-HRP to all wells

• Incubate 20 minutes at room temperature

• Wash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.

• Add 100 μL Stop solution to all wells

• Read at 450 nm within 15 minutes

Document

Technical Data Sheet