Human IFN-γ Precoated ELISA kit, 96T, CAT#1110002, BioSci

Product Description:

The human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by "sandwich one step". The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.

Product Details:

Detection range : 12.5-400 pg/mL

Sensitivity: 5 pg/mL

Precision : Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%

Stored : 2 - 8 °C

Verified Reactivity：Human

Reagents Provided and Reconstitution:

Material Required but not Provided:

•  96-well ELISA plate (microplate spectrophotometer).

•  Pipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.

•  Distilled water, fresh absorbent paper.

•  Vortex mixer and magnetic stirrer

Scientific Data:

Assay Procedure Summary:

• Add 100 μL of sample, standard or control per well
• Add 50 μL of diluted Biotinylated Detection antibody to all wells
• Incubate 2 hours at room temperature
• Wash three times
• Add 100 μL of streptavidin-HRP to all wells
• Incubate 20 minutes at room temperature
• Wash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.
• Add 100 μL Stop solution to all wells
• Read at 450 nm within 15 minutes

Document:

Technical Data Sheet