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Sku: 1110002

Product Description

The human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by "sandwich one step". The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.

 

Product Details

Detection range : 12.5-400 pg/mL

Sensitivity: 5 pg/mL

Precision : Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%

Stored : 2 - 8 °C

Verified Reactivity:Human

 

Reagents Provided and Reconstitution

Reagents Quantity Reconstitution

Cytokine standard

2 vials

Lyophilized, reconstitute with the volume of reagent indicated on the vial

Biotinylated antibody

2 vials

Dilute 50 times in Diluent buffer(1×)

Streptavidin-HRP

2 vials

Dilute 100 times in Diluent buffer(1×)

Diluent buffer(1×)

3 vials

Ready-to-use

Washing buffer(50×)

1 vial

Dilute 50 times in distilled Water

TMB

1 vial

Ready-to-use

Stop solution

1 vial

Ready-to-use

Precoated ELISA plates

8×12

Ready-to-use

Plastic cover

4 sheets

Ready-to-use

 

 

Material Required but not Provided

  • 96-well ELISA plate (microplate spectrophotometer).

  • Pipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.

  • Distilled water, fresh absorbent paper.

  • Vortex mixer and magnetic stirrer

           

          Scientific Data

           

          Assay Procedure Summary

          • Add 100 μL of sample, standard or control per well

          • Add 50 μL of diluted Biotinylated Detection antibody to all wells

          • Incubate 2 hours at room temperature

          • Wash three times

          • Add 100 μL of streptavidin-HRP to all wells

          • Incubate 20 minutes at room temperature

          • Wash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.

          • Add 100 μL Stop solution to all wells

          • Read at 450 nm within 15 minutes

           

          Document

          Technical Data Sheet

          When can I expect my order to ship?

          Most orders are filled and shipped within 2-3 business days from the time they are received.

          Our standard shipping usually take 2-5 days.

          We also provide express shippping for time-sensitive deliveries. 

          Email contact@biofargo.com if you have any requirements.

           

          Terms and Conditions

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