Streptavidin Beads Leadonano™

Description

Leadonano™ Streptavidin Magnetic Beads are superparamagnetic streptavidin-coated magnetic particles designed for rapid capture and magnetic separation of biotinylated molecules. The magnetic beads feature uniform particle size, narrow size distribution, large surface area, and defined surface coating for efficient isolation and purification workflows.

The beads are densely coated with streptavidin and blocked to support efficient binding of biotinylated proteins, antibodies, peptides, nucleic acids, and other biomolecules while maintaining high purity and reproducibility. The excellent dispersion properties and absence of magnetic remanence enable efficient liquid-phase reaction kinetics in both manual and automated workflows.

Leadonano™ Streptavidin Beads are suitable for protein interaction studies, DNA-protein pulldown assays, purification of biotin-labeled proteins and nucleic acids, immunocapture workflows, flow cytometry, and high-throughput automated applications.

Specifications

Features

• Uniform and monodispersed particles — narrow size distribution supports reproducible magnetic separation performance.

• High binding capacity — large surface area and streptavidin coating enable efficient capture of biotinylated molecules.

• Low non-specific adsorption — pre-blocked particle surface improves purification purity from complex samples.

• Rapid magnetic response — superparamagnetic ferric oxide core supports fast magnetic separation.

• Automation compatible — suitable for manual workflows, HTS systems, and automated purification platforms.

• Customizable formats — particle surface modification and product customization available for specific applications.

Additional Notes

• Irregular surface, large surface area

• Pre-blocked

• Stronger magnetic response than competing beads of the same size

• Non-porous

• Ferric oxide core functionalized with various silane groups

• Non-clumping

Applications

• Protein interaction studies — isolation and purification of biotinylated proteins and complexes

• DNA-protein pulldown assays — capture of biotin-labeled nucleic acid targets

• NGS and nucleic acid workflows — purification and magnetic separation of biotinylated DNA/RNA molecules

• Immunocapture workflows — preparation of protein-coupled magnetic beads for immunocapture applications

• Flow cytometry sample preparation — loading of desired biotinylated proteins for cell-related workflows

• Biopanning applications — magnetic enrichment and target isolation workflows

• Automated purification systems — compatible with manual and high-throughput automated workflows

Storage and Handling

This product is stable for 1 year when stored at -20 °C.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

This product is produced non-sterile.

Advantages

• Performance — high sensitivity and reproducibility support consistent experimental results.

• Efficiency — fast binding kinetics and strong magnetic response enable rapid magnetic separation.

• Purity — stable pre-blocked particles reduce non-specific adsorption from complex samples.

• Flexibility — suitable for proteins, antibodies, peptides, nucleic acids, and other biotinylated ligands.

• Automation compatibility — suitable for automated protocols and high-throughput purification workflows.

Consistency and reproducibility

The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.

Binding Capacity

The size of the ligand and the biotinylation procedure may affect the final binding capacity. Typical binding performance of Leadonano™ Streptavidin Beads includes:

• 490–750 pmol free biotin per mg of beads

• 10 µg biotinylated IgG per mg of beads

• ∼200 pmol biotinylated peptides per mg of beads

• ∼10 µg biotinylated ds-DNA per mg of beads

• ∼200 pmol biotinylated ss-oligonucleotides per mg of beads

FAQ

Q: What molecules can Leadonano™ Streptavidin Beads bind?
A: The beads are designed for binding biotinylated proteins, antibodies, peptides, nucleic acids, DNA/RNA molecules, and other biotinylated ligands.

Q: Are the beads suitable for automated purification systems?
A: Yes. The beads are suitable for both manual and automated high-throughput purification workflows.

Q: What particle sizes are available?
A: Leadonano™ Streptavidin Beads are available in 1 µm and 2.8 µm particle sizes.

Q: Can the beads be used for NGS and nucleic acid workflows?
A: Yes. The beads support purification and magnetic separation of biotinylated DNA/RNA molecules and related workflows.

Q: What is the binding capacity of the beads?
A: Typical binding performance includes 490–750 pmol free biotin per mg of beads and approximately 10 µg biotinylated IgG per mg of beads.

Q: How should the product be stored?
A: Store the product at -20 °C and avoid more than 3 freeze-thaw cycles.

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