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Sku: 6016111
Categories: Cell Culture Medium

Note:Four times the concentration of aminoacids and vitamins,low-glucose type (1000mg/L),without animal-derived components.


Product Description

Dulbecco's Modified Eagle's Medium (DMEM) is a modified Eagle Basal cell medium (BME) containing four times the concentration of amino acids and vitamins.This product is a low-glucose type (1000mg/L) and supports the cultivation of various mammalian cells, including primary fibroblasts, neurons, gliocytes, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12. This product is a GMP grade medium without animal-derived components. Therefore, additional culture additives such as fetal bovine serum (FBS) need to be supplemented when culturing cells.


Specifications

Volume 500mL
Supplementation Need additional
Glutamine L-Glutamine
Phenol Red Phenol Red
Antibiotics No Antibiotics
Sodium Pyruvate Sodium Pyruvate
Sodium Bicarbonate Sodium Bicarbonate
HEPES Buffer No HEPES
pH 7.0-7.6
Osmolality 295~335 mOsmol/kg
Endotoxin <0.3 EU/mL



Intended Use

The medium is produced by aseptic processes and can be used for further manufacturing purposes. This product is not intended for human, diagnostic or therapeutic use.


Formulation

Components (mg/L)

 Amino Acids

Glycine 30
L-Arginine•HCl 84
L-Cystine•2HCl 63
L-Glutamine 584
L-Histidine•HCl•H 2 O 42
L-Isoleucine 105
L-Leucine 105
L-Lysine•HCl 146
L-Methionine 30
L-Phenylalanine 66
L-Serine 42
L-Threonine 95
L-Tryptophan 16
L-Tyrosine•2Na•2H 2 O 104
L-Valine 94


 Vitamins

Choline chloride 4
Folic Acid 4
myo-Inositol 7.2
Niacinamide 4
D-Pantothenic Acid•1/2 Ca 4
Pyridoxal•HCl 4
Riboflavin 0.4
Thiamine•HCl 4


Inorganic Salts

CaCl₂ 200
Fe(NO₃)₃•9H₂O 0.1
MgSO₄ 97.67
KCl 400
NaHCO₃ 3700
NaCl 6400
NaH₂PO₄ 109


 Other

D-Glucose 1000
Phenol Red•Na 15
Sodium Pyruvate 110



Storage

Stored at 2℃~8℃, away from light, the shelf life is 12 months.


Notes

Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.


Document

Dulbecco’s Modified Eagle’s Medium-Low Glucose-Biosci™

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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