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Purity is ＞99% (HPLC)
$20.40
Sku: DD0056-0.5mL
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by May 05, 2026
Description
Our dNTPs are analytically tested to ensure a purity of >99% by HPLC, guaranteeing optimal performance in demanding PCR applications.
Specification
| Parameter | Specification |
|---|---|
| Purity | ≥ 99% (HPLC) |
| Total Product Size | 0.5 mL |
| Individual Container Size | 0.5 mL |
| Number of Containers | 1 |
| Refrigeration Requirements | Freezer |
| Shipping Conditions | ICE |
| UNSPSC Code | 12352208 |
| UNSPSC Category | Nucleic Acid Bases |
| Grade | Biotech |
| DG | No |
| Storage | (-15 to -20)℃ |
| Sterile | No |
| Stock | Usually in Stock |
Summary
DNase, RNase: None detected.
Suitable for use in the Polymerase Chain Reaction (PCR).
PCR Suitability
dNTP Mix is a solution containing each of the four deoxynucleotides as follows:
- 10 mM dATP
- 10 mM dCTP
- 10 mM dGTP
- 10 mM dTTP
dNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing standard PCR components. A single band of approximately 500 base pairs was visualized following electrophoresis, confirming high performance.
Quality Control
Endonuclease-Exonuclease
One μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM TrisHCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable.
Endonuclease (Nickase)
One μg of pBR322 DNA was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circularDNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.
RNase
Two μg of transfer RNA were incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.
Disclaimer
For laboratory research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
