Blood SuperDirect™ PCR Kit (EDTA)

Description

This product uses a unique PCR buffer system, without DNA extraction or sample processing, it can perform direct PCR with whole blood samples as a template. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system, and mouse blood can be up to 20%, which can sensitively amplify genomic and exogenous DNA fragments in blood samples.

2× SuperEasyTM Mix-EDTA contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the different anticoagulants, the products are divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR) Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.

Feature

• No need for time-consuming and expensive DNA purification, no pre-treatment, and direct use whole blood as a PCR template.

• The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.

• 2×SuperEasyTM Mix-EDTA has high blood tolerance: up to 45% in human blood and 20% in mouse blood.

• The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.

Application

Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).

Storage

This kit should be stored at -20°C.

• 2×SuperEasyTM Mix-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

• 6× DNA Loading Buffer, can be stored at 4℃ or -20℃ for a long time.

Documents

Blood SuperDirectTM PCR Kit-EDTA Instruction Manual