Anti-Human CD3 Monoclonal Antibody

Description

Biofargo Anti-Human CD3 Monoclonal Antibody (Clone UCHT1) is a high-specificity primary monoclonal antibody targeting the human CD3ε (epsilon) T-cell receptor complex, a key surface marker on human T lymphocytes used ubiquitously in immunology research. Designed for sensitive and reproducible detection of CD3+ T cells in flow cytometry (FACS), Western blot (WB), immunofluorescence (IF) and ELISA assays, this antibody delivers consistent performance in phenotypic analysis and functional studies of human immune cells. Formulated in a stabilized buffer with PBS and preservatives, it ensures reliable stability and activity for a wide range of immunological applications. Ideal for researchers studying human T-cell activation, immune profiling, and signaling pathways. (Primary anti-CD3 antibody, RUO grade)

Specifications

Features

• High-Specificity Targeting: Recognizes human CD3ε (epsilon) chain on T lymphocytes with high affinity, ideal for accurate T-cell identification.

• Primary Monoclonal Antibody: Mouse-derived IgG1 primary antibody with consistent performance and lot integrity for research use.

• Multi-Application Use: Validated for Flow Cytometry (FACS), Western Blot, ELISA, and Immunofluorescence, facilitating versatile immunological experiments.

• Stable Formulation: Supplied in phosphate buffered saline (PBS) with stabilizers (BSA and sodium azide), optimized for long-term stability.

• Research-Grade Quality: RUO (Research Use Only) antibody trusted by labs for immunophenotyping, T-cell activation studies, and cellular signaling research.

Applications

• Flow Cytometry (FACS): Precise quantification and profiling of human CD3+ T cells in PBMCs and tissue samples.

• Western Blot (WB): Detection of CD3ε protein expression in human cell lysates.

• Immunofluorescence (IF): Visualization of T-cell distribution and localization in cultured cells or tissue.

• ELISA & Immunoassays: Analyzing CD3 presence or modulation in functional immunology studies.

• T-Cell Activation & Signaling: Used in research to stimulate or monitor T-cell receptor interactions and downstream pathways.

Storage 

Stable for up to 24 months when stored at 2-8°C under sterile condition.

Documents

Data Sheet

SDS

References

1. Silvia M Bacot, Taylor A Harper, Rebecca L Matthews, Christie Jane Fennell, Adovi Akue, Mark A KuKuruga, Shiowjen Lee, Tao Wang, Gerald M Feldman. Exploring the Potential Use of a PBMC-Based Functional Assay to Identify Predictive Biomarkers for Anti-PD-1 Immunotherapy. Int J Mol Sci. 2020 Nov 27;21(23):9023.

2. Min Zhou, Jing Wang, Cui-Ping Li, Jing-Yan Xu, Bing Chen. Autologous Cytokine-Induced Killer Cell Immunotherapy for Patients with High-Risk Diffuse Large B Cell Lymphoma After the First Complete Remission. Onco Targets Ther. 2020 Jun 22;13:5879-5885.

Attention

For research and manufacturing purposes only.