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Sku: 143-2
Categories: PCR

 Intended use

  • The 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe.

  • The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.


Product Characteristics

  • The kit is designed for singleplex RT-PCR with TaqMan probe.

  • For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.

  • The RTase is easily heat-inactivated at ≥90°C for 1min.

  • The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.

  • The RTase reversely-transcribes single digit copies of target RNA molecules consistently.

  • The kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve.

  • The concentrations of the primers and probe are variable depending on assay designs and thermocycling protocols (User Manual Table 1).

  • The preferable PCR product size is ≤150bp.

  • The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.



User Manual

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.


Terms and Conditions

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