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Sku: RT-PCR 143-2
Categories: Dlab PCR

Intended use

  • The 1-Step 2X RT-PCR Master Mix-TaqMan probe is used for real-time qualitative and quantitative RT-PCR with TaqMan probe.

  • The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.


  • For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.

  • The RTase efficiently synthesizes a complementary DNA strand on RNA template, ≤1 unit per 20 μL of reaction.

  • The RTase can detect single digit copies of target RNA molecules.

  • The kit also contains Taq-Probe DNA polymerase for the amplification step, specially engineered for TaqMan probes and generating S-Shaped curves.

  • The kit is designed for singleplex RT-PCR.

  • The preferable PCR product size is ≤150bp.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.


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