1-Step 2X Fast RT-PCR Master Mix-SYBR Green, #Cat. RT-PCR 147

Intended Use

• The 1-Step 2X Fast RT-PCR Master Mix is used for qualitative and quantitative RT-PCR amplifications with SYBR Green dye for fast thermocycling.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Kit Characterizations

• For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.

• The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.

• The RTase can detect single digit copies of target RNA molecules.

• The kit also contains Taq-Fast DNA polymerase for the amplification step, which extends more than 300 bases with short cycling program.

• The kit is designed for singleplex RT-PCR.

• The preferable PCR product size is ≤150bp.