Cell Culture Dilution / Seeding Density Calculator

Calculate the volume of cell suspension needed to reach a target seeding density, using C1V1 = C2V2.

Stock cell concentration — C1 (cells/mL)

Leave exactly one of the four fields below blank to solve for it.

Stock volume needed — V1 (mL)

Target seeding density — C2 (cells/mL)

Final culture volume — V2 (mL)

Determine your stock concentration (C1) with a hemocytometer or automated cell counter before using this calculator.