Description

Biofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: T25, T75, and T175.

Specifications

Colour	Flask: clear
Colour	Cap: Red
Material	Flask: PS
Material	Cap: PP
Sterilization	Irradiation sterilization
Volume	Growth area: 75cm²
Volume	Max. volume: 250mL
Treatment	TC
Quality Control	Raw Material-Control: physical testing
	Product-Control: testing of attributive and variable
	characteristics in accordance with the valid specification

Features

TC treated
Vented caps with 0.2μm filter, promoting ventilation and preventing contamination.
Angled bottle neck designed for pipettes and cell scrapers.
Measurements and annotation area on both sides.
Sterilized self-sealing bag to prevent contamination.
Easily stackable.
Gamma sterilized, DNase-free, RNase-free.

Applications

TC-Treated (Adherent Treatment):

Scenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.

Effect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.

Frequently Asked Questions

What is a T75 cell culture flask used for?

A T75 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 75 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.

What does TC-treated mean on a cell culture flask?

TC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.

What is the growth area of a T75 flask?

A T75 flask provides a growth surface area of 75 cm² and a maximum volume of 250 mL. This size is commonly used for routine cell expansion and offers a balance between culture capacity and laboratory handling convenience.

What is the recommended working volume for a T75 cell culture flask?

Although the maximum volume is 250 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.

What is the difference between a vented cap and a sealed cap cell culture flask?

A vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.

Why is a 0.2 μm vent filter important in cell culture flasks?

The 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.

Can this T75 flask be used for HEK293, CHO, Vero, or HeLa cells?

Yes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.

Are Biofargo T75 cell culture flasks sterile?

Yes. Biofargo T75 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.

Are these cell culture flasks DNase-free and RNase-free?

Yes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.

What material is used to manufacture the flask?

The flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.

Can these T75 flasks be stacked during incubation?

Yes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.

What types of applications are T75 tissue culture flasks commonly used for?

T75 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.

T75 Cell Culture Flasks with Vented Cap TC Treated Sterile