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Description
YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.
Components
Ingredients (g/L)
Yeast Extract 10.0g
Peptone 20.0g
D-glucose 20.0g
Application
YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.
Usage
50 g per liter
Reconstitution
Preparation instructions
-
Suspend 50 g in 1 L of distilled water.
-
Autoclave for 15 minutes at 121 °C.
Documents
Safety information
-
Storage Class
11 - Combustible Solids
-
Wgk_germany
WGK 3
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Flash_point_f
Not applicable
-
Flash_point_c
Not applicable
Tag： Yeast Extract Peptone Dextrose Broth
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