Ficoll 400-Biofargo

Description

Ficoll 400: Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications.

Specifications

Application

Suitable for

• hybridization experiments to reduce non-specific binding of labeled probes to the membrane

• preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF

• separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)

• producing density gradients to separate subcellular components

• vitrification of cell and tissue samples.

• to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Used for cell separation and organelle isolation.

Features

• free from DNases, RNases, phosphatases and proteases

• preserves functional and morphological integrity of the separated cells

Documents

COA

SDS

Disclaimer: For laboratory research use only.