Xanthomonas campestris pv. ricini Probe Realtime PCR Kit

Description

Xanthomonas campestris pv. ricini Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Precision disease diagnostics in castor bean cultivation: rapid, on-site qPCR screening of irrigation water and leaf tissue for early detection of X. campestris pv. ricini to support integrated pest management and minimize yield losses
• Pathogen surveillance for seed certification and trade compliance: quantitative monitoring of bacterial load in seed lots and nursery stock to ensure regulatory standards, reduce quarantine risks and guarantee product quality
• Research on host–pathogen interactions and resistance breeding: high-sensitivity quantification of X. campestris pv. ricini in greenhouse trials and field studies for evaluation of cultivar resistance, biocontrol efficacy and novel disease management strategies

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Xanthomonas campestris pv. ricini Probe Realtime PCR Kit User Manual

MSDS