Xanthomonas campestris pv. cerealis Probe Realtime PCR Kit

Description

Xanthomonas campestris pv. cerealis Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Seed Health Testing in Cereal Breeding and Certification Programs: Quantitative monitoring of Xanthomonas campestris pv. cerealis in wheat and barley seeds to ensure pathogen-free seed lots and compliance with international phytosanitary standards.
• Plant Pathology and Disease Resistance Research in Agricultural Biotechnology: Sensitive, high-throughput detection for host–pathogen interaction studies, screening of resistance germplasm, and mechanistic investigations of bacterial leaf streak in cereals.
• Agricultural Biosecurity and Crop Protection Surveillance: Routine field and post-harvest monitoring of cereal crops for early warning, epidemiological tracking, and integration into precision agriculture platforms to support timely disease management and food safety strategies.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Xanthomonas campestris pv. cerealis Probe Realtime PCR Kit User Manual

MSDS