Wolbachia spp. Probe Realtime PCR Kit

Description

Wolbachia spp. Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Aquaculture Health Management: Routine high-sensitivity screening of Wolbachia in abalone, shrimp and finfish broodstock enhances disease surveillance, supports biosecurity protocols, and informs selective breeding for improved yield and product safety.
• Environmental & Marine Ecology: Quantitative detection of Wolbachia in water and sediment samples drives ecosystem health monitoring, biodiversity assessments, and compliance with environmental regulations in marine biology and conservation studies.
• Vector Biology & Biocontrol Development: Accurate quantification of Wolbachia in crustacean and insect vectors accelerates research on host–symbiont interactions, symbiont-based pathogen blocking, and novel vector control strategies for public health applications.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Wolbachia spp. Probe Realtime PCR Kit User Manual

MSDS