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All-in-One Probe RT-PCR Kit for Sensitive and Rapid Detection of Pichia pastoris – 50 Reactions
$456.00
Sku: 15-58900
Description
Pichia pastoris Probe RT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.
Key Features
One-tube qPCR format minimizes carry-over contamination risk
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High sensitivity: analytical LOD as low as 100 copies/reaction
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Excellent specificity: no cross-reactivity with DNA from other pathogens
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Includes amplification positive control for result verification
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Compatible with various DNA purification methods
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Suitable for both qualitative and quantitative applications
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For quantitative use: the linear range covers a 100,000-fold concentration difference.
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Sufficient for 50 reactions (20 μL each)
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For Research Use Only (RUO)
Kit Components
| Component Name | Product Number. | Size | Vial | Lid Color |
| Probe qRT-PCR Buffer | 990504a | 500 μL | 0.5 μL Vial | Blue |
| Probe qRT-PCR Enzyme | 990504bv2 | 50 μL | 0.5 mL Vial | Red |
| Template Dilution Buffer | 180701 | 1 mL | 1.5 mL Vial | Green |
| Primer-Probe Powder | yp15-58900 | 50 T | 1.5 mL Brown Vial | Brown |
| Amplification Positive Control (1E7 copies/μL) |
pc58900 | 0.5 mL | 0.5 mL Vial | Yellow |
| Manual | sc15-58900 | 1 Set | NA | NA |
Application Areas
- Biopharmaceutical and fermentation process control: rapid, quantitative monitoring of Pichia pastoris contamination during upstream and downstream processing, in-process controls, and release testing of recombinant protein production batches.
- Industrial biotechnology and strain management: quality control for recombinant enzyme and protein manufacturers, including strain verification, detection of cross-contamination between production strains, and cell bank testing.
- Research and laboratory applications: routine screening for P. pastoris contamination in molecular biology and microbiology labs, verification of engineered strains, and quantitative assays for experimental workflows.
Storage & Shipping
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Store at - 20 °C
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The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
FAQ
Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?
A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.
PCR Kits can be used with standard thermal cyclers from leading brands.
Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.
Our technical team is available to assist with instrument setup and run optimization to ensure the best results.
Q: Can this kit be used for digital droplet PCR (ddPCR)?
A: Yes.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
