Actinomyces neuii radingae Probe Realtime PCR Kit

Description

Actinomyces neuii radingae Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Compatible with various DNA purification methods

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Clinical microbiology and infectious disease research: quantitative detection of Actinomyces neuii and Actinomyces radingae in human clinical specimens (e.g., wound/abscess material, blood, urine, tissue, oral swabs) for infection surveillance, epidemiology and research applications (For Research Use Only, not for diagnostic use).
• Oral and dental research: monitoring A. neuii and A. radingae abundance in oral microbiome studies, periodontal disease research, implant-associated infection investigations and evaluation of antimicrobial treatments.
• Microbiome and infection-control studies: quantitative analysis of Actinomyces species in microbiome profiling, hospital/clinical environment surveillance and research on colonization dynamics to support outbreak investigations and antimicrobial stewardship efforts.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Actinomyces neuii radingae Probe Realtime PCR Kit User Manual

MSDS