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$72.00
Sku: TAP-211
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 13, 2026
Description
Taq DNA polymerase is the most widely used thermostable DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq) YT-1. The enzyme possesses a 5’→3’ polymerase activity and a double-strand specific 5’→ 3’ exonuclease activity.
Features
- • Tolerates various kinds of PCR protocols.
- • Applicable for hot start technology by adding anti-Taq antibody (Code No. TCP-101).
- • PCR products can be cloned by using a TA cloning method.
- • Incorporates dUTP, dITP, and fluorescently-labeled nucleotides.
Application
-
PCR
-
Primer extension
Specification
| Category | Item | Details |
|---|---|---|
| Source | - | E. coli strain carrying the cloned Taq DNA polymerase gene from Thermus aquaticus (Taq) YT-1. |
| Unit definition | - | One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP into an acid insoluble material in 30 min at 75ºC. |
| Storage condition | - | 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 0.5% Nonidet™ P-40, 0.5% Tween™ 20, 50% Glycerol. Store at -20ºC |
This reagent includes the following components for 100-200 reactions;
Components
| Item |
|---|
| rTaq DNA Polymerase (2.5U/µL) |
| 10×Buffer |
| 25 mM MgCl2 |
| 2mM dNTPs |
Typical PCR Reaction Setup
Normal PCR
| Component | Volume | Final Concentration |
|---|---|---|
| 10x Buffer | 5 µL | 1× |
| 2mM dNTPs | 5 µL | 0.2 mM each |
| 25mM MgCl2 | 3 µL | 1.5 mM |
| 10pmol/ul Primer #1 | 1.0 µL | 0.2 µM |
| 10pmol/ul Primer #2 | 1.0 µL | 0.2 µM |
| Template DNA | X µL | Genomic DNA 10~1000 ng/50 µL Plasmid DNA 1~50 ng/50 µL cDNA ~200 ng (RNA equiv.)/50 µL |
| PCR grade water | Y µL | |
| Diluted rTth DNA polymerase (1.0U/µL) | 1.25-2.5 µL | 1.25-2.5 U / 50 µL |
| Total reaction volume | 50 µL |
PCR Cycle Conditions
*Extension time should be set at 1 min per 1 kb of target length.
Application Data
Example 1.Amplification of 180 bp–1.3 kb genes from human genomic DNA
Distinct and specific amplified bands from 180 bp to 1.3 kb were observed with rTaq DNA polymerase by 1% agarose gel electrophoresis.
References
-
F.C. Lawyer, S. Stoffel, R.K. Saiki, K. Myambo, R. Drummond, D.H. Gelfand., J. Biol. Chem., 264: 6427-6437 (1989)
-
T. Nagahama, K. Sugiura, S. Lee, H. Morita, Y. Adachi, A.H. Kwon, Y. Kamiyama, S. Ikehara, Stem cells, 19: 425-435 (2001)
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
