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Description
FGF-2 (Fibroblast Growth Factor-2 (Basic)), also known as bFGF, is one of the earliest recognized members of the FGF family. Secreted by endothelial cells, smooth muscle cells, and macrophages, FGF-2 acts on a variety of cell types. As a crucial regulator of cell growth and differentiation, FGF-2 possesses biological activities such as pro-angiogenic, proliferative, chemotactic, and migratory effects, playing a vital role in processes of cell differentiation and organismal development.
Specifications
| Product Details | |
|---|---|
| Code | Y00341 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL. |
| Accession | XP_030252529.1 |
| Alternative Names | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
| Species | Sparus aurata |
| Expression System | E. coli |
| Mol. Weight | 17.1 kDa |
| Expression Region | Met1-Cys155 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | 10 mM PB, 250 mM NaCl, 5% mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at-20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Data Figures

Figure 1: Recombinant Sparus aurata bFGF (Cat. No. Y00341) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is >2.0 ng/mL. The bioactivity of recombinant Sparus aurata bFGF (Cat. No. Y00341) was higher than the other competing product.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

