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$220.00
Sku: TL-783-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
Wnt3a is a member of the Wnt family and is involved in cell self-renewal, proliferation, differentiation, and cell movement.
The classical Wnt/β-catenin signaling pathway is one of the most conserved pathways in evolution and plays a key role in embryonic development, cell growth, differentiation, polarity formation, neural development, and carcinogenesis.
Wnt3a is also a commonly used cytokine in organoid culture.
In the culture of intestinal organoids, the addition of Wnt3a helps support the proliferation and self-renewal of intestinal stem cells.
Wnt3a can also promote the proliferation and differentiation of neural precursor cells, thereby supporting the maturation and function of neural organoids.
Specifications
| Parameter | Specification |
|---|---|
| Expression Host | HEK293 cells |
| Synonyms | Wnt-3a; Wnt 3a; WNT3A; Wnt Surrogate-Fc Fusion |
| Protein Sequence | Amino acid sequence: Met1–Lys352 of human Wnt3a (UniProt ID: P56704-1) with hFc (IgG1) tag at the C-Terminus |
| Molecular Weight | Predicted molecular weight is 58.5 kDa |
| Purity | >95%, analyzed by SDS-PAGE |
| Endotoxin | <1 EU/μg |
| Purification Method | Chromatographic purification |
| Appearance | White lyophilized powder |
| Activity | Refer to COA for details |
Features
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HEK293-Expressed Human Wnt3a-Fc Fusion Protein
Recombinant human Wnt3a corresponding to Met1–Lys352 fused with a C-terminal human IgG1 Fc tag, expressed in HEK293 cells with a predicted molecular weight of 58.5 kDa.
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High Purity Chromatographic Preparation
Purity >95% confirmed by SDS-PAGE following chromatographic purification.
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Controlled Endotoxin Level
Endotoxin level <1 EU/μg suitable for in vitro cell culture and signaling studies.
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Defined Lyophilized Protein Format
Supplied as a white lyophilized powder with verified electrophoretic identity and consistent protein quality.
Applications
Wnt/β-Catenin Signaling Research
Activates the canonical Wnt signaling pathway involved in embryonic development, cell proliferation, differentiation, and polarity regulation.
Intestinal Organoid Culture
Supports proliferation and self-renewal of intestinal stem cells during intestinal organoid culture.
Neural Organoid Development
Promotes proliferation and differentiation of neural precursor cells, facilitating neural organoid maturation and functional studies.
Stem Cell Culture Systems
Used in stem cell culture environments including MSC and iPSC research models.
Storage
Store at -20 ℃
Instructions
Centrifuge before reopening the tube. Dissolve the lyophilized protein in distilled water. It is recommended to reconstitute the concentration to greater than 100 μg /mL.
After reconstitution, the protein can be stored at -80℃ for 3-6 months and at 2-8℃ for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.
Precautions
This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
