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$306.00
Sku: Y03001-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
LIF (Leukemia Inhibitory Factor) is a pleiotropic cytokine belonging to the IL-6 family. As a secreted glycoprotein, it activates downstream signaling pathways by binding to the LIFR/gp130 receptor complex and plays a critical role in cell culture, particularly in stem cell maintenance. Its primary function is to sustain pluripotency in mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). In other stem cell contexts, LIF promotes the survival and proliferation of neural stem cells and, in combination with factors such as SCF and IL-3, helps maintain the stemness of hematopoietic stem cells.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y03001 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/mL. |
| Accession | P15018 |
| Alternative Names | Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 19.7 kDa |
| Expression Region | Ser23-Phe202 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Maintains hematopoietic progenitor survival in vitro.
Immunology
Supports certain immune cell subsets (e.g., Tregs) indirectly.
Stem Cell Research
Widely used to maintain pluripotency of ESCs and support stem cell survival.
Cell Biology
Regulates cell survival, proliferation, and differentiation via JAK/STAT3 signaling.
Data Figures
Figure 1: Recombinant Human LIF (Cat. No. Y03001) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03001 SDS-PAGE.
Figure 2: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/mL.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
